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Literature summary for 4.2.1.51 extracted from
- Prephenate dehydratase of the actinomycete Amycolatopsis methanolica: purification and characterization of wild-type and deregulated mutant proteins (1995), Biochem. J., 308, 313-320.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-methionine | 1 mM, 1.6fold activation | Amycolatopsis methanolica |  |
| L-tyrosine | Ka: 410 mM | Amycolatopsis methanolica | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-phenylalanine | 1 mM, 48% inhibition, Ki: 670 mM | Amycolatopsis methanolica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
4 * 34000, SDS-PAGE | Amycolatopsis methanolica |
| 150000 | - |
gel filtration | Amycolatopsis methanolica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate | Amycolatopsis methanolica | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amycolatopsis methanolica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Amycolatopsis methanolica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33.3 | - |
- |
Amycolatopsis methanolica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 40% v/v glycerol | Amycolatopsis methanolica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate | - |
Amycolatopsis methanolica | phenylpyruvate + H2O + CO2 | - |
? | |
| prephenate | - |
Amycolatopsis methanolica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 34000, SDS-PAGE | Amycolatopsis methanolica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Amycolatopsis methanolica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 670 | - |
L-phenylalanine | - |
Amycolatopsis methanolica | |
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