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Literature summary for 4.2.1.51 extracted from
- Chorismate mutase-prephenate dehydratase from Escherichia coli (1987), Methods Enzymol., 142, 432-439.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli |  |
| L-phenylalanine | one phenylalanine-binding site per subunit | Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| tetrathionate | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | 1 | prephenate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43111 | - |
2 * 43111, smallest native species of enzyme, calculation from nucleotide sequence | Escherichia coli |
| 43111 | - |
8 * 43111, calculation from nucleotide sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate | Escherichia coli | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| bifunctional enzyme, chorismate mutase:prephenate dehydratase | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 169 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20 mM Tris-HCl, pH 8.2, 1 mM EDTA, 10 mM 2-mercaptoethanol, 0.3 M NaCl, 0.02% w/v NaN3, 1 mM dihiothreitol, 2 weeks stable | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate | - |
Escherichia coli | phenylpyruvate + H2O + CO2 | - |
? | |
| prephenate | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43111, smallest native species of enzyme, calculation from nucleotide sequence | Escherichia coli |
| octamer | 8 * 43111, calculation from nucleotide sequence | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.6 | - |
Escherichia coli |
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