Any feedback?
Literature summary for 4.2.1.53 extracted from
- Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia (2012), J. Biotechnol., 158, 17-23.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tween 40 | maximum activity at 0.05% (v/v) Tween 40 | Stenotrophomonas maltophilia |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566, BL21(DE3), W3110, and MLK1067 cells | Stenotrophomonas maltophilia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
oleate | at pH 6.5 and 35°C | Stenotrophomonas maltophilia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
2 * 68000, SDS-PAGE | Stenotrophomonas maltophilia |
| 136000 | - |
gel filtration | Stenotrophomonas maltophilia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleate + H2O | Stenotrophomonas maltophilia | highest specific activity | (R)-10-hydroxystearate | 98% yield after 4 h | ? | |
| oleate + H2O | Stenotrophomonas maltophilia KCTC 1773 | highest specific activity | (R)-10-hydroxystearate | 98% yield after 4 h | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Stenotrophomonas maltophilia | - |
- |
- |
| Stenotrophomonas maltophilia KCTC 1773 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap column chromatography | Stenotrophomonas maltophilia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.014 | - |
with myristoleic acid as substrate, at pH 6.5 and 35°C | Stenotrophomonas maltophilia |
| 0.033 | - |
with gamma-linolenic acid as substrate, at pH 6.5 and 35°C | Stenotrophomonas maltophilia |
| 0.54 | - |
with alpha-linolenic acid as substrate, at pH 6.5 and 35°C | Stenotrophomonas maltophilia |
| 0.91 | - |
with linoleic acid as substrate, at pH 6.5 and 35°C | Stenotrophomonas maltophilia |
| 1.95 | - |
with palmitoleic acid as substrate, at pH 6.5 and 35°C | Stenotrophomonas maltophilia |
| 3.69 | - |
with oleate as substrate, at pH 6.5 and 35°C | Stenotrophomonas maltophilia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-linolenic acid + H2O | - |
Stenotrophomonas maltophilia | 10-hydroxy-12,15(Z,Z)-octadecadienoic acid | - |
? | |
| alpha-linolenic acid + H2O | - |
Stenotrophomonas maltophilia KCTC 1773 | 10-hydroxy-12,15(Z,Z)-octadecadienoic acid | - |
? | |
| gamma-linolenic acid + H2O | - |
Stenotrophomonas maltophilia | 10-hydroxy-6,12(Z,Z)-octadecadienoic acid | - |
? | |
| gamma-linolenic acid + H2O | - |
Stenotrophomonas maltophilia KCTC 1773 | 10-hydroxy-6,12(Z,Z)-octadecadienoic acid | - |
? | |
| linoleic acid + H2O | - |
Stenotrophomonas maltophilia | 10-hydroxy-12(Z)-octadecenoic acid | - |
? | |
| additional information | no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid | Stenotrophomonas maltophilia | ? | - |
? | |
| additional information | no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid | Stenotrophomonas maltophilia KCTC 1773 | ? | - |
? | |
| myristoleic acid + H2O | - |
Stenotrophomonas maltophilia | 10-hydroxymyristic acid | - |
? | |
| oleate + H2O | highest specific activity | Stenotrophomonas maltophilia | (R)-10-hydroxystearate | 98% yield after 4 h | ? | |
| oleate + H2O | highest specific activity | Stenotrophomonas maltophilia KCTC 1773 | (R)-10-hydroxystearate | 98% yield after 4 h | ? | |
| Palmitoleic acid + H2O | - |
Stenotrophomonas maltophilia | 10-Hydroxypalmitic acid | - |
? | |
| Palmitoleic acid + H2O | - |
Stenotrophomonas maltophilia KCTC 1773 | 10-Hydroxypalmitic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 68000, SDS-PAGE | Stenotrophomonas maltophilia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Stenotrophomonas maltophilia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.83 | - |
oleate | at pH 6.5 and 35°C | Stenotrophomonas maltophilia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Stenotrophomonas maltophilia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the addition of FAD (0.01-0.2 mM) has no effect on the activity | Stenotrophomonas maltophilia |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25.67 | - |
oleate | at pH 6.5 and 35°C | Stenotrophomonas maltophilia | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
