Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 0.01 M, 30% inhibition | Rhodospirillum rubrum | |
iodoacetic acid | 0.01 M, 12% inhibition | Rhodospirillum rubrum | |
N-ethylmaleimide | 0.01 M, 23% inhibition | Rhodospirillum rubrum | |
p-Substituted mercuribenzoate | 3 mM, 65% inhibition | Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00926 | - |
crotonyl-CoA | - |
Rhodospirillum rubrum | |
0.0263 | - |
trans-2-hexenoyl-CoA | - |
Rhodospirillum rubrum | |
0.08 | - |
crotonyl-[acyl-carrier protein] | - |
Rhodospirillum rubrum | |
0.118 | - |
Crotonylpantetheine | - |
Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA | Rhodospirillum rubrum | enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodospirillum rubrum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O | stereochemistry | Rhodospirillum rubrum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-3-hydroxybutanoyl-CoA | - |
Rhodospirillum rubrum | crotonyl-CoA + H2O | - |
? | |
crotonyl-CoA | enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria | Rhodospirillum rubrum | ? | - |
? | |
Crotonyl-CoA + H2O | - |
Rhodospirillum rubrum | (3R)-3-Hydroxybutanoyl-CoA | - |
? | |
Crotonyl-[acyl-carrier protein] + H2O | about 50% of the activity with crotonyl-CoA | Rhodospirillum rubrum | ? | - |
? | |
Crotonylpantetheine | about 55% of the activity with crotonyl-CoA | Rhodospirillum rubrum | ? | - |
? | |
additional information | no activity with crotonylglutathione, acrylyl-CoA | Rhodospirillum rubrum | ? | - |
? | |
trans-2-Hexenoyl-CoA + H2O | about 35% of the activity with crotonyl-CoA | Rhodospirillum rubrum | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.6 | - |
Rhodospirillum rubrum |