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Literature summary for 4.2.1.65 extracted from

  • Castric, P.A.; Farnden, J.F.; Conn, E.E.
    Cyanide metabolism in higher plants. V. The formation of asparagine from beta-cyanoalanine (1972), Arch. Biochem. Biophys., 152, 62-69.
    View publication on PubMed
Search for more literature for 4.2.1.65

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
 Lupinus angustifolius
HgCl2
-
 Lupinus angustifolius
iodoacetic acid
-
 Lupinus angustifolius
N-ethylmaleimide
-
 Lupinus angustifolius
NaCN
-
 Lupinus angustifolius
Zn(CH3COO)2
-
 Lupinus angustifolius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 Beta-cyano-L-alanine
-
 Lupinus angustifolius

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Lupinus angustifolius
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
400000 500000 gel filtration Lupinus angustifolius

Organism

Organism UniProt Comment Textmining
Lupinus angustifolius
-
-
-
Sorghum bicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lupinus angustifolius

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Lupinus angustifolius
-
root
-
 Lupinus angustifolius
-
seedling
-
 Lupinus angustifolius
-
shoot
-
 Lupinus angustifolius
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.371
-
-
 Sorghum bicolor
0.371
-
-
 Lupinus angustifolius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-Cyano-L-alanine + H2O
-
 Sorghum bicolor L-Asparagine
-
 ?
beta-Cyano-L-alanine + H2O
-
 Lupinus angustifolius L-Asparagine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Lupinus angustifolius

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
 Lupinus angustifolius