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Literature summary for 4.2.1.70 extracted from
- Studies on the biosynthesis of 5-ribosyluracil 5'-monophosphate in Tetrahymena pyriformis (1964), J. Biol. Chem., 239, 1177-1187.
General Stability
| General Stability | Organism |
|---|---|
| activity retained after freezing and thawing | Tetrahymena pyriformis |
| buffers containing 20% glycerol stabilize the enzyme | Tetrahymena pyriformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
ribose 5-phosphate | - |
Tetrahymena pyriformis |  |
| 0.071 | - |
Uracil | - |
Tetrahymena pyriformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, broad maximal activity between 0.01 M and 0.05 M | Tetrahymena pyriformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetrahymena pyriformis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Tetrahymena pyriformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.2 | - |
- |
Tetrahymena pyriformis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 1 year without loss of activity | Tetrahymena pyriformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-thiouracil + D-ribose 5-phosphate | - |
Tetrahymena pyriformis | ? | - |
? | |
| isocytosine + D-ribose 5-phosphate | - |
Tetrahymena pyriformis | ? | - |
? | |
| uracil + D-ribose 5-phosphate | - |
Tetrahymena pyriformis | pseudouridine 5'-phosphate + H2O | - |
? | |
| uracil + ribose 5-phosphate | r | Tetrahymena pyriformis | 5-ribosyluracil 5'-monophosphate + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Tetrahymena pyriformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | 37 | about 50% of maximal activity at 25°C and about 30% of maximal activity at 37°C | Tetrahymena pyriformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Tetrahymena pyriformis |
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Release 2023.1 (January 2023)
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