Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-malate | weak | Pseudomonas sp. | |
EDTA | Fe2+ or Co2+ restores; strong | Pseudomonas sp. | |
L(+)-Tartrate | weak | Pseudomonas sp. | |
L-malate | weak | Pseudomonas sp. | |
meso-tartrate | - |
Pseudomonas sp. | |
additional information | no inhibition by iodoacetate | Pseudomonas sp. | |
Zn2+ | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
(S,S)-Tartrate | strain Ch | Pseudomonas sp. | |
0.6 | - |
(S,S)-Tartrate | strain G2 | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pseudomonas sp. | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requirement, can replace Fe2+ or Co2+ with about 30% (strain G2) and 55% (strain Ch) efficiency | Pseudomonas sp. | |
Co2+ | requirement, 0.5 mM, equally effective as Fe2+ | Pseudomonas sp. | |
divalent cations | requirement | Pseudomonas sp. | |
Fe2+ | requirement, 0.1 mM, equally effective as Co2+ | Pseudomonas sp. | |
Mg2+ | requirement, can replace Fe2+ or Co2+ with about 60% (strain G2) and 80% (strain Ch) efficiency | Pseudomonas sp. | |
Mn2+ | requirement, can replace Fe2+ or Co2+ with about 65% efficiency | Pseudomonas sp. | |
Ni2+ | requirement, can replace Fe2+ or Co2+ with about 30% (strain G2) and 80% (strain Ch) efficiency | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
2 * 38500, SDS-PAGE | Pseudomonas sp. |
72000 | - |
strain Ch, gel filtration | Pseudomonas sp. |
78000 | - |
strain G2, gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S,S)-tartrate | Pseudomonas sp. | - |
oxaloacetate + H2O | - |
? | |
additional information | Pseudomonas sp. | involved in D-tartrate catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
strains G2 and Ch | - |
Oxidation Stability | Organism |
---|---|
insensitive to O2, no thiols required for protection, 15 h stable at 22°C | Pseudomonas sp. |
Purification (Comment) | Organism |
---|---|
to near homogeneity | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
strain Ch | Pseudomonas sp. |
13.6 | - |
strain G2 | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S,S)-tartrate | - |
Pseudomonas sp. | oxaloacetate + H2O | - |
? | |
additional information | involved in D-tartrate catabolism | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38500, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas sp. |