Activating Compound | Comment | Organism | Structure |
---|---|---|---|
light | restores activity inhibited by NO | Rhodococcus sp. | |
potassium hexacyanoferrate | oxidizing agent that activates the Co-substituted enzyme by oxidizing the Co2+ atom and/or modification of a alphaCys-112 to cysteine-sulfinic acid | Rhodococcus sp. |
Application | Comment | Organism |
---|---|---|
synthesis | useful for acrylamide production | Rhodococcus sp. |
Cloned (Comment) | Organism |
---|---|
Co-substituted NHase produced in Escherichia coli grown in Co supplemented medium | Rhodococcus sp. |
expression in Escherichia coli | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NO | reversible inhibition, for recombinant enzyme | Rhodococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | incorporated instead of Fe into the catalytic site with initial weak activity that increases when an oxidizing agent is added | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | alphaCys112 is modified to a cysteine-sulfinic acid in both recombinant and native enzymes | Rhodococcus sp. |
Purification (Comment) | Organism |
---|---|
chromatography on butyl-toyopearl | Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
97 | - |
purified co-substituted enzyme | Rhodococcus sp. |