Literature summary for 4.2.2.1 extracted from

Mello, L.V.; De Groot, B.L.; Li, S.; Jedrzejas, M.J.
Structure and flexibility of Streptococcus agalactiae hyaluronate lyase complex with its substrate. Insights into the mechanism of processive degradation of hyaluronan (2002), J. Biol. Chem., 277, 36678-36688.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of the enzymes from strains 3502 and 4755 in Escherichia coli	Streptococcus agalactiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme-hyaluronate hexasaccharide substrate complex, hanging drop vapour diffusion method, room temperature, in 10-50 mM hyaluronate hexasaccharide, 0.1 M sodium cacodylate, pH 6.0, 30 mM potassium thiocyanate, 30% PEG monomethyl ester 5000, several days, X-ray diffraction structure determination and analysis at 2.2 A resolution	Streptococcus agalactiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
92000	-	x * 92000, SDS-PAGE	Streptococcus agalactiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
hyaluronan	Streptococcus agalactiae	elimination of the polymer in the host connective tissue	3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus agalactiae	Q53591	strains 3502, gene hyl3502, and 4755, gene hyl4755	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli	Streptococcus agalactiae

Reaction

Reaction	Comment	Organism	Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate	catalytic mechanism, the flexibility of the enzyme structure is important, the beta-domain is involved in the progressive catalytic process, enzyme substrate interactions	Streptococcus agalactiae	a

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hyaluronan	-	Streptococcus agalactiae	3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine	unsaturated disaccharide	?
hyaluronan	elimination of the polymer in the host connective tissue	Streptococcus agalactiae	3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine	-	?
hyaluronate hexasaccharide	-	Streptococcus agalactiae	3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine	unsaturated disaccharide	?

Subunits

Subunits	Comment	Organism
?	x * 92000, SDS-PAGE	Streptococcus agalactiae
More	enzyme is composed of 2 domains, alpha-helical alpha-domain and beta-sheet beta-domain, which are structurally and functionally distinct	Streptococcus agalactiae

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Release 2023.1 (January 2023)