Literature summary for 4.2.2.1 extracted from

Rigden, D.J.; Littlejohn, J.E.; Joshi, H.V.; de Groot, B.L.; Jedrzejas, M.J.
Alternate structural conformations of Streptococcus pneumoniae hyaluronan lyase: insights into enzyme flexibility and underlying molecular mechanism of action (2006), J. Mol. Biol., 358, 1165-1178.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant fully active truncated enzyme form composed of the catalytic and C-terminal domains by two different method resulting in different crystal forms, 5 mg/ml protein in 10 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 2 mM EDTA, hanging drop vapour diffusion method, mixing with an equal volume of reservoir solution containing 1. 30% PEG 2000 monomethyl ester, 0.1 M MES, pH 6.5, and 0.2 M ammonium sulfate, or 2. 70% w/v saturated malonic acid, pH 7.6, and 0.1 M sodium cacodylate, ph 6.6, several months, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular dynamic simulations	Streptococcus pneumoniae

Crystallization (Comment)

Organism

purified recombinant fully active truncated enzyme form composed of the catalytic and C-terminal domains by two different method resulting in different crystal forms, 5 mg/ml protein in 10 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 2 mM EDTA, hanging drop vapour diffusion method, mixing with an equal volume of reservoir solution containing 1. 30% PEG 2000 monomethyl ester, 0.1 M MES, pH 6.5, and 0.2 M ammonium sulfate, or 2. 70% w/v saturated malonic acid, pH 7.6, and 0.1 M sodium cacodylate, ph 6.6, several months, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular dynamic simulations

Streptococcus pneumoniae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a fully active truncated enzyme form composed of the catalytic and C-terminal domains	Streptococcus pneumoniae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell surface	-	Streptococcus pneumoniae	9986	-
cell surface	-	Streptococcus agalactiae	9986	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
hyaluronic acid	Streptococcus pneumoniae	degradation of hyaluronate in connective tissue of hosts to facilitate invasion	hyaluronic acid oligomers	-	?
hyaluronic acid	Streptococcus agalactiae	degradation of hyaluronate in connective tissue of hosts to facilitate invasion	hyaluronic acid oligomers	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus agalactiae	Q53591	-	-
Streptococcus pneumoniae	Q54873	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate	active site structure and hydrophobic patch, molecular mechanism, enzyme flexibility and structural requirements	Streptococcus pneumoniae	a
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate	active site structure and hydrophobic patch, molecular mechanism, enzyme flexibility and structural requirements	Streptococcus agalactiae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
chondroitin sulfate	degradation	Streptococcus pneumoniae	?	-	?
chondroitin sulfate	degradation	Streptococcus agalactiae	?	-	?
hyaluronan	degradation	Streptococcus pneumoniae	hyaluronic acid oligomers	-	?
hyaluronan	degradation	Streptococcus agalactiae	hyaluronic acid oligomers	-	?
hyaluronic acid	degradation of hyaluronate in connective tissue of hosts to facilitate invasion	Streptococcus pneumoniae	hyaluronic acid oligomers	-	?
hyaluronic acid	degradation of hyaluronate in connective tissue of hosts to facilitate invasion	Streptococcus agalactiae	hyaluronic acid oligomers	-	?
unsulfated chondroitin	degradation	Streptococcus pneumoniae	?	-	?
unsulfated chondroitin	degradation	Streptococcus agalactiae	?	-	?

Subunits

Subunits	Comment	Organism
More	structure analysis, active site structure and hydrophobic patch, structure-function relationship	Streptococcus pneumoniae
More	three-dimensional structure analysis, active site structure and hydrophobic patch, structure-function relationship	Streptococcus agalactiae

Synonyms

Synonyms	Comment	Organism
Hyal	-	Streptococcus pneumoniae
Hyal	-	Streptococcus agalactiae