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Literature summary for 4.2.2.2 extracted from

  • Sato, M.; Kaji, A.
    Purification and properties of pectate lyase produced by Streptomyces fradiae IFO 3439 (1975), Agric. Biol. Chem., 39, 819-824.
No PubMed abstract available

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, optimal concentration varies with levels of the substrate Streptomyces fradiae

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
IFO 3439
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces fradiae

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Streptomyces fradiae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
176
-
-
Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hexagalacturonic acid 17.2% of the activity with polygalacturonic acid Streptomyces fradiae ?
-
?
additional information macerating activity on Ganpi bark, carrot, radish and sweet potato Streptomyces fradiae ?
-
?
pentagalacturonic acid 5.9% of the activity with polygalacturonic acid Streptomyces fradiae saturated digalacturonic acid + unsaturated trigalacturonic acid + saturated trigalacturonic acid + unsaturated digalacturonic acid
-
?
polygalacturonic acid random attack Streptomyces fradiae unsaturated oligogalacturonate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 6.0-9.0, 3 h, stable Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.2
-
Streptomyces fradiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable at 2°C for 72 h or at 30°C for 3 h Streptomyces fradiae