Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.0022 mM, 50% inhibition | Thermobifida fusca | |
Cd2+ | - |
Thermobifida fusca | |
dithiothreitol | 0.0104 mM, 50% inhibition | Thermobifida fusca | |
Hg2+ | - |
Thermobifida fusca | |
Pb2+ | - |
Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km-value for pectin increases 30000fold as the percent esterification of the substrate increases from 0 to 60% | Thermobifida fusca |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermobifida fusca | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Thermobifida fusca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Oxidation Stability | Organism |
---|---|
bubbling O2 through the enzyme preparation increases activity 63% | Thermobifida fusca |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | contains 0.195 mg of glucose equivalents per mg of protein | Thermobifida fusca |
Purification (Comment) | Organism |
---|---|
- |
Thermobifida fusca |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Thermobifida fusca | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin | - |
Thermobifida fusca | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Ca2+ slightly enhances thermal stability | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Thermobifida fusca |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
2-mercaptoethanol | - |
Thermobifida fusca | |
0.0104 | - |
dithiothreitol | - |
Thermobifida fusca |