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Literature summary for 4.2.2.2 extracted from
- Comparative analysis of the five major Erwinia chrysanthemi pectate lyases: enzyme characteristics and potential inhibitors (1997), J. Bacteriol., 179, 2503-2511.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Dickeya chrysanthemi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Dickeya chrysanthemi |  |
| Ca2+ | 1 mM, strong inhibition of isoenzyme pelE | Dickeya chrysanthemi | |
| Co2+ | - |
Dickeya chrysanthemi | |
| epicatechin | - |
Dickeya chrysanthemi | |
| Mn2+ | 1 mM, weak | Dickeya chrysanthemi | |
| salicylic acid | - |
Dickeya chrysanthemi | |
| Sr2+ | 1 mM, weak | Dickeya chrysanthemi | |
| Zn2+ | - |
Dickeya chrysanthemi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Dickeya chrysanthemi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Dickeya chrysanthemi | |
| Ca2+ | optimal concentration: 0.1 mM | Dickeya chrysanthemi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39500 | - |
x * 39500, isoenzymes PelB and PelC, SDS-PAGE | Dickeya chrysanthemi |
| 42000 | - |
x * 42000, isoenzyme PelD, SDS-PAGE | Dickeya chrysanthemi |
| 42500 | - |
x * 42500, isoenzymes PelA and PelE, SDS-PAGE | Dickeya chrysanthemi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
5 isoenzymes: PelA, PelB, PelC, PelD, PelE | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 5 isoenzymes: PelA, PelB, PelC, PelD, PelE | Dickeya chrysanthemi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Dickeya chrysanthemi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid | - |
Dickeya chrysanthemi | unsaturated oligogalacturonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42500, isoenzymes PelA and PelE, SDS-PAGE | Dickeya chrysanthemi |
| ? | x * 42000, isoenzyme PelD, SDS-PAGE | Dickeya chrysanthemi |
| ? | x * 39500, isoenzymes PelB and PelC, SDS-PAGE | Dickeya chrysanthemi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
isoenzyme PelD and pelE | Dickeya chrysanthemi |
| 55 | - |
isoenzyme, isoenzyme A | Dickeya chrysanthemi |
| 60 | - |
isoenzyme PelB pelC | Dickeya chrysanthemi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half life: 11 min for isoenzyme PelA, 4 min for isoenzyme PelD, 2 min for isoenzyme PelE | Dickeya chrysanthemi |
| 60 | - |
half-life: 20-30 min for isoenzyme PelB and PelC, isoenzymes PelA, PelD and PelE loses more than 80% of activity after 2 min | Dickeya chrysanthemi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
isoenzyme pelE | Dickeya chrysanthemi |
| 8.5 | - |
isoenzyme PelA | Dickeya chrysanthemi |
| 8.8 | - |
isoenzyme isoenzyme pelD | Dickeya chrysanthemi |
| 9.2 | - |
isoenzyme PelC | Dickeya chrysanthemi |
| 9.3 | - |
isoenzyme PelB | Dickeya chrysanthemi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Dickeya chrysanthemi |
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