Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, strong | Bacillus sp. (in: Bacteria) | |
Ba2+ | 1 mM, strong | Bacillus sp. (in: Bacteria) | |
Cd2+ | 1 mM, moderate | Bacillus sp. (in: Bacteria) | |
Cu2+ | 1 mM, moderate | Bacillus sp. (in: Bacteria) | |
EDTA | activity is recovered by addition of Ca2+ | Bacillus sp. (in: Bacteria) | |
Mn2+ | 1 mM, moderate | Bacillus sp. (in: Bacteria) | |
Zn2+ | 1 mM, strong | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for polygalacturonic acid is 0.85 mg/ml | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
TS 47 | - |
Bacillus sp. (in: Bacteria) TS 47 | - |
TS 47 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
pectin | - |
Bacillus sp. (in: Bacteria) TS 47 | ? | - |
? | |
polygalacturonic acid | - |
Bacillus sp. (in: Bacteria) | unsaturated galacturonate | - |
? | |
polygalacturonic acid | - |
Bacillus sp. (in: Bacteria) TS 47 | unsaturated galacturonate | - |
? | |
protopectin | - |
Bacillus sp. (in: Bacteria) | pectin + ? | - |
? | |
protopectin | - |
Bacillus sp. (in: Bacteria) TS 47 | pectin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
endo-polygalacturonate trans-eliminase | - |
Bacillus sp. (in: Bacteria) |
PL | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 75 | 50°C: about 20% of maximal activity, 60°C: about 45% of maximal activity, 75°C: about 75% of maximal activity, 80°C: about 10% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
half-life: 13 h | Bacillus sp. (in: Bacteria) |
70 | - |
half-life: 1 h | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | pH 7.0: about 35% of maximal activity, pH 8.0: optimum, pH 9.0: no activity | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | isoelectric focusing | - |
5.3 |