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Literature summary for 4.2.2.2 extracted from

  • Takao, M.; Nakaniwa, T.; Yoshikawa, K.; Terashita, T.; Sakai, T.
    Purification and characterization of thermostable pectate lyase with protopectinase activity from thermophilic Bacillus sp. TS 47 (2000), Biosci. Biotechnol. Biochem., 64, 2360-2367.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, strong Bacillus sp. (in: Bacteria)
Ba2+ 1 mM, strong Bacillus sp. (in: Bacteria)
Cd2+ 1 mM, moderate Bacillus sp. (in: Bacteria)
Cu2+ 1 mM, moderate Bacillus sp. (in: Bacteria)
EDTA activity is recovered by addition of Ca2+ Bacillus sp. (in: Bacteria)
Mn2+ 1 mM, moderate Bacillus sp. (in: Bacteria)
Zn2+ 1 mM, strong Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for polygalacturonic acid is 0.85 mg/ml Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
TS 47
-
Bacillus sp. (in: Bacteria) TS 47
-
TS 47
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin
-
Bacillus sp. (in: Bacteria) ?
-
?
pectin
-
Bacillus sp. (in: Bacteria) TS 47 ?
-
?
polygalacturonic acid
-
Bacillus sp. (in: Bacteria) unsaturated galacturonate
-
?
polygalacturonic acid
-
Bacillus sp. (in: Bacteria) TS 47 unsaturated galacturonate
-
?
protopectin
-
Bacillus sp. (in: Bacteria) pectin + ?
-
?
protopectin
-
Bacillus sp. (in: Bacteria) TS 47 pectin + ?
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
endo-polygalacturonate trans-eliminase
-
Bacillus sp. (in: Bacteria)
PL
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 75 50°C: about 20% of maximal activity, 60°C: about 45% of maximal activity, 75°C: about 75% of maximal activity, 80°C: about 10% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
half-life: 13 h Bacillus sp. (in: Bacteria)
70
-
half-life: 1 h Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
7 8 pH 7.0: about 35% of maximal activity, pH 8.0: optimum, pH 9.0: no activity Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) isoelectric focusing
-
5.3