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Literature summary for 4.2.2.2 extracted from

  • Sawada, K.; Ogawa, A.; Ozawa, T.; Sumitomo, N.; Hatada, Y.; Kobayashi, T.; Ito, S.
    Nucleotide and amino-acid sequences of a new-type pectate lyase from an alkaliphilic strain of Bacillus (2000), Eur. J. Biochem., 267, 1510-1515.
    View publication on PubMed
Search for more literature for 4.2.2.2

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, pH 7.5, 10 min at 30°C, activity is completely abolished, fully recovered by adding 0.8 mM CaCl2 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolute requirement Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q9XDT2
-
-
Bacillus sp. (in: Bacteria) KSM-P15 Q9XDT2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Bacillus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin relative degradation rates of citrus pectin with methylation degrees 31%, 63% and 94% is 124%, 73% and 9% compared to polygalacturonic acid Bacillus sp. (in: Bacteria) ?
-
 ?
pectin relative degradation rates of citrus pectin with methylation degrees 31%, 63% and 94% is 124%, 73% and 9% compared to polygalacturonic acid Bacillus sp. (in: Bacteria) KSM-P15 ?
-
 ?
polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) unsaturated oligogalacturonides
-
 ?
polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) KSM-P15 unsaturated oligogalacturonides
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
pectate lyase
-
 Bacillus sp. (in: Bacteria)
pectate lyase E
-
 Bacillus sp. (in: Bacteria)
pectate transeliminase
-
 Bacillus sp. (in: Bacteria)
Pel-15E
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 40°C: about 50% of maximal activity, 60°C: about 25% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 Ca2+ increases thermal stability of the enzyme slightly Bacillus sp. (in: Bacteria)
60
-
 pH 7.5, stable in presence of Ca2+ Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
8.5 11.5 pH 8.5: about 35% of maximal activity, pH 11.5: 75% of maximal activity Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) isoelectric focusing, pH range: 3-10
-
 9.2