Literature summary for 4.2.2.2 extracted from

Hatada, Y.; Saito, K.; Koike, K.; Yoshimatsu, T.; Ozawa, T.; Kobayashi, T.; Ito, S.
Deduced amino-acid sequence and possible catalytic residues of a novel pectate lyase from an alkaliphilic strain of Bacillus (2000), Eur. J. Biochem., 267, 2268-2275.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
D106N	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
D126N	the ratio of turnover number to Km-value is 57.5% of the wild-type value	Bacillus sp. (in: Bacteria)
D63N	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
D80N	the ratio of turnover number to Km-value is 93% of the wild-type value	Bacillus sp. (in: Bacteria)
D84N	the ratio of turnover number to Km-value is 28.6% of the wild-type value	Bacillus sp. (in: Bacteria)
E38Q	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
E47Q	mutant enzyme retains almost full activity relative to wild-type enzyme	Bacillus sp. (in: Bacteria)
E83Q	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
H66A	the ratio of turnover number to Km-value is 70.4% of the wild-type value	Bacillus sp. (in: Bacteria)
K107A	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
K107H	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
K107R	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
K129A	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
K129H	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
K129R	mutant enzyme is not produced extracellularly	Bacillus sp. (in: Bacteria)
K182A	mutant enzyme retains almost full activity relative to wild-type enzyme	Bacillus sp. (in: Bacteria)
K185A	mutant enzyme retains almost full activity relative to wild-type enzyme	Bacillus sp. (in: Bacteria)
K20A	mutant enzyme retains almost full activity relative to wild-type enzyme	Bacillus sp. (in: Bacteria)
K41A	the ratio of turnover number to Km-value is 105% of the wild-type value	Bacillus sp. (in: Bacteria)
K89A	the ratio of turnover number to Km-value is 31.5% of the wild-type value	Bacillus sp. (in: Bacteria)
additional information	chimeric enzyme composed of Ala1 to Tyr105 of Pel-15 in the N-terminal regions, Asp133 to Arg 159 of pectate lyase B from Fusarium solani in the internal regions, and Gln133 to Tyr197 of Pel-15 in the C-terminal regions: the ratio of turnover number to Km-value is 5.1% of the wild-type value	Bacillus sp. (in: Bacteria)
R132A	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
R132H	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
R132K	mutant enzyme without pectate lyase activity	Bacillus sp. (in: Bacteria)
R152A	mutant enzyme retains almost full activity relative to wild-type enzyme	Bacillus sp. (in: Bacteria)
W78F	the ratio of turnover number to Km-value is identical to wild-type value	Bacillus sp. (in: Bacteria)
W78Y	mutant enzyme with slightly increased activity relative to wild-type enzyme	Bacillus sp. (in: Bacteria)
Y174A	the ratio of turnover number to Km-value is 90.7% of the wild-type value	Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	KM-values for wild-type and mutant enzymes for polygalacturonic acid: 1.87 g/l for wild-type enzyme, 1.55 g/l for mutant enzyme K41A, 1.9 g/l for mutant enzyme H66A, 2.07 g/l for mutant enzyme W78F, 1.78 g/l for mutant enzyme D80N, 1.6 g/l for mutant enzyme D84N, 1.42 g/l for mutant enzyme K89A, 1.34 g/l for mutant enzyme D126N, 1.86 g/l for mutant enzyme Y174A, 1.55 g/l for the chimeric enzyme composed of Ala1 to Tyr105 of Pel-15 in the N-terminal regions, Asp133 to Arg 159 of pectate lyase B from Fusarium solani in the internal regions, and Gln133 to Tyr197 of Pel-15 in the C-terminal regions	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	Q9RHW0	KSM-P15	-
Bacillus sp. (in: Bacteria) KSM-P15	Q9RHW0	KSM-P15	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
200	220	-	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polygalacturonic acid	-	Bacillus sp. (in: Bacteria)	unsaturated oligogalacturonide	-	?
polygalacturonic acid	-	Bacillus sp. (in: Bacteria) KSM-P15	unsaturated oligogalacturonide	-	?

Synonyms

Synonyms	Comment	Organism
Pel-15	-	Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.03	0.55	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme chimeric enzyme composed of Ala1 to Tyr105 of Pel-15 in the N-terminal regions, Asp133 to Arg 159 of pectate lyase B from Fusarium solani in the internal regions, and Gln133 to Tyr197 of Pel-15 in the C-terminal regions	Bacillus sp. (in: Bacteria)
7.48	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme chimeric enzyme composed of Ala1 to Tyr105 of Pel-15 in the N-terminal regions, Asp133 to Arg 159 of pectate lyase B from Fusarium solani in the internal regions, and Gln133 to Tyr197 of Pel-15 in the C-terminal regions	Bacillus sp. (in: Bacteria)
42.2	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme K89A	Bacillus sp. (in: Bacteria)
43.2	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme D84N	Bacillus sp. (in: Bacteria)
72.9	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme D126N	Bacillus sp. (in: Bacteria)
101	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme H66A	Bacillus sp. (in: Bacteria)
154	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme K41A	Bacillus sp. (in: Bacteria)
156	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme D80N	Bacillus sp. (in: Bacteria)
159	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme Y126N	Bacillus sp. (in: Bacteria)
173	-	polygalacturonic acid	pH 10.5, 30°C, wild-type enzyme	Bacillus sp. (in: Bacteria)
196	-	polygalacturonic acid	pH 10.5, 30°C, mutant enzyme W78F	Bacillus sp. (in: Bacteria)