Application | Comment | Organism |
---|---|---|
biotechnology | enhancing PGL production by controlling the optimal ratio provides an alternative approach to enhance heterologous protein production with Pichia pastoris | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Pichia pastoris. The ratio of methanol to cell concentration has a significant influence on PGL production. An advanced glycerol exponential feeding strategy is developed for biomass accumulation in cell growth phase, by which cell concentration reaches 140 g/L after 19 h glycerol feeding. In subsequent production phase, a methanol feeding profile is proposed according to the optimal ratio of methanol to cell concentration at a range of 0.163-0.171 g/g, and PGL activity and productivity reaches 430 U/ml and 4.34 U/ml/h, respectively | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonate | - |
Bacillus subtilis | unsaturated polygalacturonic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline polygalacturonate lyase | - |
Bacillus subtilis |
PGL | - |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
assay at | Bacillus subtilis |