Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Origami cells | Streptomyces thermocarboxydus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Streptomyces thermocarboxydus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23900 | - |
mature enzyme, electrospray ionization mass spectrometry | Streptomyces thermocarboxydus |
23960 | - |
mature enzyme, calculated from amino acid sequence | Streptomyces thermocarboxydus |
28000 | - |
recombinant His-tagged enzyme, SDS-PAGE | Streptomyces thermocarboxydus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces thermocarboxydus | C6L867 | - |
- |
Streptomyces thermocarboxydus B1 | C6L867 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Streptomyces thermocarboxydus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.03 | - |
purified recombinant enzyme | Streptomyces thermocarboxydus |
177 | - |
purified native enzyme | Streptomyces thermocarboxydus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonate | - |
Streptomyces thermocarboxydus | unsaturated 4,5-digalacturonate + unsaturated 4,5-trigalacturonate | - |
? | |
polygalacturonate | - |
Streptomyces thermocarboxydus B1 | unsaturated 4,5-digalacturonate + unsaturated 4,5-trigalacturonate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PelA | - |
Streptomyces thermocarboxydus |
PL I | an endo-acting family 3 pectate lyase | Streptomyces thermocarboxydus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | inactivation of recombinant PL I occurs above 30°C whereas the activity of native PL I is stable up to 40°C | Streptomyces thermocarboxydus |