Any feedback?
Literature summary for 4.2.2.2 extracted from
- Microbial pectate lyases: Characterization and enzymological properties (2009), World J. Microbiol. Biotechnol., 25, 1-14.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Dickeya chrysanthemi |
| expressed in Escherichia coli | Bacillus licheniformis |
| expressed in Escherichia coli | Niveispirillum irakense |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | strong inhibition | Alkalihalobacillus alcalophilus |  |
| Ba2+ | strong inhibition | Niveispirillum irakense | |
| Co2+ | strong inhibition | Bacillus pumilus | |
| EDTA | strong inhibition | Bacillus pumilus | |
| epicatechin | - |
Dickeya chrysanthemi | |
| Mn2+ | strong inhibition | Alkalihalobacillus alcalophilus | |
| Mn2+ | weakly inhibits PelA activity at 1 mM | Dickeya chrysanthemi | |
| additional information | Mg2+ and Zn2+ have no effect on Pel-15 activity | Bacillus sp. (in: Bacteria) | |
| salicylic acid | - |
Dickeya chrysanthemi | |
| Sr2+ | weakly inhibits PelA activity at 1 mM | Dickeya chrysanthemi | |
| Zn2+ | strong inhibition | Niveispirillum irakense | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
galacturonan | - |
Dickeya chrysanthemi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | optimum Ca2+ concentration at 0.2 mM | Pseudonocardia sp. | |
| Ca2+ | optimum Ca2+ concentration at 0.2 mM | Niveispirillum irakense | |
| Ca2+ | optimum Ca2+ concentration at 0.5 mM | Pseudomonas fluorescens | |
| Ca2+ | optimum Ca2+ concentration at 0.5 mM | Dickeya chrysanthemi | |
| Ca2+ | optimum Ca2+ concentration at 0.6 mM | Bacillus licheniformis | |
| Ca2+ | optimum Ca2+ concentration at 0.6 mM | Fusarium verticillioides | |
| Ca2+ | optimum Ca2+ concentration at 2.0 mM | Bacillus subtilis | |
| Ca2+ | optimum Ca2+ concentration at 2.0-4.0 mM | Cellvibrio japonicus | |
| Ca2+ | optimum concentration at 0.5 mM | Pseudomonas viridiflava | |
| Ca2+ | optimum concentration at 0.6 mM | Bacillus sp. (in: Bacteria) | |
| Ca2+ | optimum concentration at 0.6 mM | Dickeya chrysanthemi | |
| Ca2+ | optimum concentration at 1.0 mM | Pseudomonas marginalis | |
| Ca2+ | optimum concentration at 1.0 mM, Ca2+ cannot be replaced by Ba2+, Be2+, Sr2+, Mg2+, and monovalent cations for Pel activity | Fusarium solani | |
| additional information | no requirement of Ca2+ | Bacillus pumilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10600 | - |
ultracentrifugation | Fusarium verticillioides |
| 11000 | - |
gel filtration | Fusarium verticillioides |
| 12100 | - |
SDS-PAGE | Fusarium verticillioides |
| 23000 | - |
SDS-PAGE | Bacillus subtilis |
| 31000 | - |
SDS-PAGE | Pseudonocardia sp. |
| 33400 | - |
SDS-PAGE | Bacillus licheniformis |
| 35000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 35000 | - |
SDS-PAGE | Alkalihalobacillus alcalophilus |
| 37300 | - |
SDS-PAGE | Bacillus pumilus |
| 42000 | - |
SDS-PAGE | Bacillus subtilis |
| 42000 | - |
SDS-PAGE | Clostridium cellulovorans |
| 42500 | - |
isoform PelA, SDS-PAGE | Dickeya chrysanthemi |
| 44000 | - |
SDS-PAGE | Dickeya chrysanthemi |
| 44400 | - |
SDS-PAGE | Niveispirillum irakense |
| 50000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 56000 | - |
SDS-PAGE | Thermobifida fusca |
| 68500 | - |
SDS-PAGE | Cellvibrio japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alkalihalobacillus alcalophilus | - |
- |
- |
| Bacillus licheniformis | - |
- |
- |
| Bacillus pumilus | - |
- |
- |
| Bacillus pumilus BK2 | - |
- |
- |
| Bacillus sp. (in: Bacteria) | - |
- |
- |
| Bacillus sp. (in: Bacteria) KSM-P15 | - |
- |
- |
| Bacillus sp. (in: Bacteria) P4-N | - |
- |
- |
| Bacillus sp. (in: Bacteria) TS 47 | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis 168 | - |
- |
- |
| Bacillus subtilis SO113 | - |
- |
- |
| Cellvibrio japonicus | - |
- |
- |
| Clostridium cellulovorans | - |
- |
- |
| Dickeya chrysanthemi | - |
- |
- |
| Dickeya chrysanthemi | P04959 | isoform PelB | - |
| Dickeya chrysanthemi | P0C1A2 | - |
- |
| Dickeya chrysanthemi | P11073 | isoform PelC | - |
| Dickeya chrysanthemi | P18209 | isoform PelD | - |
| Fusarium solani | - |
- |
- |
| Fusarium verticillioides | - |
- |
- |
| Niveispirillum irakense | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas marginalis | - |
- |
- |
| Pseudomonas viridiflava | - |
- |
- |
| Pseudonocardia sp. | - |
- |
- |
| Thermobifida fusca | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Niveispirillum irakense |
| affinity column chromatography and gel filtration | Fusarium verticillioides |
| Ni-NTA column chromatography | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin | - |
Bacillus pumilus | unsaturated tetragalacturonate | - |
? | |
| citrus pectin | - |
Bacillus pumilus BK2 | unsaturated tetragalacturonate | - |
? | |
| galacturonan | - |
Dickeya chrysanthemi | ? | - |
? | |
| pectin | - |
Bacillus subtilis | ? | - |
? | |
| pectin | - |
Pseudomonas fluorescens | ? | - |
? | |
| pectin | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| pectin | - |
Bacillus licheniformis | ? | - |
? | |
| pectin | - |
Dickeya chrysanthemi | ? | - |
? | |
| pectin | - |
Pseudonocardia sp. | ? | - |
? | |
| pectin | - |
Pseudomonas viridiflava | ? | - |
? | |
| pectin | - |
Pseudomonas marginalis | ? | - |
? | |
| pectin | - |
Fusarium solani | ? | - |
? | |
| pectin | - |
Thermobifida fusca | ? | - |
? | |
| pectin | - |
Fusarium verticillioides | ? | - |
? | |
| pectin | - |
Alkalihalobacillus alcalophilus | ? | - |
? | |
| pectin | - |
Cellvibrio japonicus | ? | - |
? | |
| pectin | - |
Niveispirillum irakense | ? | - |
? | |
| pectin | - |
Clostridium cellulovorans | ? | - |
? | |
| pectin | - |
Bacillus subtilis 168 | ? | - |
? | |
| pectin | - |
Bacillus subtilis SO113 | ? | - |
? | |
| pectin | - |
Bacillus sp. (in: Bacteria) TS 47 | ? | - |
? | |
| pectin | - |
Bacillus sp. (in: Bacteria) KSM-P15 | ? | - |
? | |
| pectin | - |
Bacillus sp. (in: Bacteria) P4-N | ? | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus subtilis | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Pseudomonas fluorescens | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus sp. (in: Bacteria) | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Dickeya chrysanthemi | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Pseudonocardia sp. | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Pseudomonas viridiflava | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Pseudomonas marginalis | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Fusarium solani | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Thermobifida fusca | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Fusarium verticillioides | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Alkalihalobacillus alcalophilus | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Cellvibrio japonicus | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Niveispirillum irakense | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Clostridium cellulovorans | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus subtilis 168 | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus subtilis SO113 | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus sp. (in: Bacteria) TS 47 | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus sp. (in: Bacteria) KSM-P15 | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus sp. (in: Bacteria) P4-N | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus pumilus | unsaturated tetragalacturonate | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus pumilus BK2 | unsaturated tetragalacturonate | - |
? | |
| polygalacturonate | pectate lyase cleaves the alpha-1,4 glycosidic bonds of polygalacturonate via a beta-elimination reaction | Bacillus licheniformis | unsaturated trigalacturonate + unsaturated oligogalacturonates | major product | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pel | - |
Bacillus subtilis |
| Pel | - |
Bacillus sp. (in: Bacteria) |
| Pel | - |
Pseudomonas viridiflava |
| Pel | - |
Pseudomonas marginalis |
| Pel | - |
Fusarium solani |
| Pel | - |
Thermobifida fusca |
| Pel-15 | - |
Bacillus sp. (in: Bacteria) |
| PelA | - |
Bacillus subtilis |
| PelA | - |
Pseudomonas fluorescens |
| PelA | - |
Bacillus sp. (in: Bacteria) |
| PelA | - |
Bacillus licheniformis |
| PelA | - |
Bacillus pumilus |
| PelA | - |
Pseudonocardia sp. |
| PelA | - |
Fusarium verticillioides |
| PelA | - |
Alkalihalobacillus alcalophilus |
| PelA | - |
Cellvibrio japonicus |
| PelA | - |
Niveispirillum irakense |
| PelA | - |
Clostridium cellulovorans |
| PelA | - |
Dickeya chrysanthemi |
| PelA | isoform | Dickeya chrysanthemi |
| PelB | isoform | Dickeya chrysanthemi |
| pelC | isoform | Dickeya chrysanthemi |
| PelD | isoform | Dickeya chrysanthemi |
| PelE | isoform | Dickeya chrysanthemi |
| PelI | - |
Dickeya chrysanthemi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacillus subtilis |
| 40 | - |
- |
Niveispirillum irakense |
| 45 | - |
- |
Alkalihalobacillus alcalophilus |
| 50 | - |
- |
Clostridium cellulovorans |
| 50 | - |
- |
Dickeya chrysanthemi |
| 50 | - |
isoform PelD | Dickeya chrysanthemi |
| 50 | - |
isoform PelE | Dickeya chrysanthemi |
| 55 | - |
isoform PelA | Dickeya chrysanthemi |
| 60 | - |
- |
Thermobifida fusca |
| 60 | - |
isoform PelB | Dickeya chrysanthemi |
| 60 | - |
isoform PelC | Dickeya chrysanthemi |
| 62 | - |
- |
Cellvibrio japonicus |
| 65 | - |
- |
Bacillus subtilis |
| 65 | - |
- |
Bacillus licheniformis |
| 70 | - |
- |
Bacillus sp. (in: Bacteria) |
| 70 | - |
- |
Bacillus pumilus |
| 70 | - |
- |
Pseudonocardia sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus sp. (in: Bacteria) |
| 8 | - |
- |
Clostridium cellulovorans |
| 8 | - |
isoform PelE | Dickeya chrysanthemi |
| 8.4 | - |
- |
Bacillus subtilis |
| 8.5 | - |
- |
Bacillus licheniformis |
| 8.5 | - |
- |
Bacillus pumilus |
| 8.5 | - |
- |
Fusarium verticillioides |
| 8.5 | - |
isoform PelA | Dickeya chrysanthemi |
| 8.6 | - |
- |
Dickeya chrysanthemi |
| 8.8 | - |
isoform PelD | Dickeya chrysanthemi |
| 9 | - |
- |
Niveispirillum irakense |
| 9 | 10 | - |
Alkalihalobacillus alcalophilus |
| 9.2 | - |
isoform PelC | Dickeya chrysanthemi |
| 10 | - |
- |
Bacillus subtilis |
| 10 | - |
- |
Cellvibrio japonicus |
| 10.3 | - |
- |
Pseudonocardia sp. |
| 10.5 | - |
- |
Thermobifida fusca |
| 11.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermobifida fusca | isoelectric focusing | - |
4.2 |
| Dickeya chrysanthemi | isoelectric focusing | - |
4.6 |
| Dickeya chrysanthemi | isoform PelA, isoelectric focusing | - |
4.6 |
| Bacillus sp. (in: Bacteria) | isoelectric focusing | - |
5.3 |
| Niveispirillum irakense | isoelectric focusing | - |
6.2 |
| Pseudomonas fluorescens | minor pI, isoelectric focusing | - |
6.7 |
| Dickeya chrysanthemi | isoform PelB, isoelectric focusing | - |
7.9 |
| Dickeya chrysanthemi | isoform PelC, isoelectric focusing | - |
8.2 |
| Bacillus pumilus | isoelectric focusing | - |
8.5 |
| Bacillus subtilis | isoelectric focusing | - |
8.6 |
| Fusarium verticillioides | isoelectric focusing | - |
9.1 |
| Bacillus subtilis | isoelectric focusing | - |
9.6 |
| Bacillus sp. (in: Bacteria) | isoelectric focusing | - |
9.6 |
| Pseudomonas viridiflava | isoelectric focusing | - |
9.7 |
| Dickeya chrysanthemi | isoform PelD, isoelectric focusing | - |
9.8 |
| Pseudonocardia sp. | isoelectric focusing | - |
10 |
| Pseudomonas fluorescens | major pI, isoelectric focusing | - |
10 |
| Dickeya chrysanthemi | isoform PelE, isoelectric focusing | - |
10.3 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
