Cloned (Comment) | Organism |
---|---|
gene Pcpel2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli BL21(DE3) using the pMAL or pLysS system, expression of maltose binding fusion protein, MBP-PCPEL2 | Phytophthora capsici |
Crystallization (Comment) | Organism |
---|---|
purified recombinant PCPEL2, hanging-drop vapour-diffusion method, 16°C, 0.001 ml of protein solution containing 5 mg/ml protein in 10 mM Tris-HCl, pH 7.5, 150 mM NaCl and 1 mM DTT, is mixed with 0.001 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, 10% w/v PEG 6000, 5% v/v 2-methy-2,4-pentanediol, equilibration versus 0.2 ml of reservoir solution, 5 days, X-ray diffraction structure determination and analysis | Phytophthora capsici |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phytophthora capsici | G2XKV7 | gene Pcpel2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged PCPEL2 from Escherichia coli strain BL21(DE3), 34.1fold from the pLysS system and 53.8fold from the pMal system, by nickel affinity chromatography and gel filtration chromatography, recombinant MBP-PCPEL2 by amylose affinity chromatography | Phytophthora capsici |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
750 | - |
purified recombinant PCPEL2 expressed from pLysS, pH not specified in the publication, temperature not specified in the publication | Phytophthora capsici |
860 | - |
purified recombinant PCPEL2 expressed from pMAL, pH not specified in the publication, temperature not specified in the publication | Phytophthora capsici |
Synonyms | Comment | Organism |
---|---|---|
PCPEL2 | - |
Phytophthora capsici |