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Literature summary for 4.2.2.2 extracted from

  • Wang, H.; Li, X.; Ma, Y.; Song, J.
    Characterization and high-level expression of a metagenome-derived alkaline pectate lyase in recombinant Escherichia coli (2014), Process Biochem., 49, 69-76.
No PubMed abstract available
Search for more literature for 4.2.2.2

Application

Application Comment Organism
environmental protection alkaline pectate lyases play an important role in mild and eco-friendly bioscouring pretreatment processes in the textile industry. So far, only a few pectate lyases can be applied in industrial-scale production, and many of them exhibit high production cost, low activity, and/or do not meet the treatment requirements. Recombinant PelB has with promising properties for use in bioscouring in the textile pretreatment process and is a potential enzyme for industrial applications uncultured bacterium

Cloned(Commentary)

Cloned (Comment) Organism
gene pelB, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ strong inhibition at 0.5-1 mM uncultured bacterium
Cu2+ strong inhibition at 1 mM uncultured bacterium
Fe2+ strong inhibition at 1 mM uncultured bacterium
Mg2+ strong inhibition at 1 mM uncultured bacterium
Mn2+ strong inhibition at 0.5-1 mM uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, activates 5.8fold at 0.5 mM uncultured bacterium
Cu2+ activates slightly at 0.5 mM uncultured bacterium
K+ activates slightly at 0.5-1 mM uncultured bacterium
Mg2+ activates slightly at 0.5 mM uncultured bacterium
Triton X-100 activates slightly at 0.5-1 mM uncultured bacterium
Tween-20 activates slightly at 1 mM uncultured bacterium
Zn2+ activates slightly at 0.5-1 mM uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium S5UBI8 gene pelB cloned from the metagenomic DNA of alkaline environment soils
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 2.47fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured bacterium

Source Tissue

Source Tissue Comment Organism Textmining
additional information high cell-density cultivation in 7-L bioreactor, expression profile over time, overview uncultured bacterium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
614.7
-
 purified recombinant His-tagged enzyme, 50-70% methylated pectin, pH 9.8, 45°C uncultured bacterium
668.2
-
 purified recombinant His-tagged enzyme, over 80% methylated pectin, pH 9.8, 45°C uncultured bacterium
1984
-
 purified recombinant His-tagged enzyme, unmethylated polygalacturonic acid, pH 9.8, 45°C uncultured bacterium
2096
-
 purified recombinant His-tagged enzyme, 20-34% methylated polygalacturonic acid, pH 9.8, 45°C uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PelB is an endo-acting lyase and shows high cleavage capability on a broad range of substrates of natural methylated pectin, substrate specificity, overview uncultured bacterium ?
-
 ?
pectin methylated at low-degree uncultured bacterium ?
-
 ?
polygalacturonic acid best substrate uncultured bacterium ? final major end products are dimers, trimers, and tetramers of unsaturated galacturonic acid, PelB does not produce any monomeric GalpA ?

Synonyms

Synonyms Comment Organism
alkaline pectate lyase
-
 uncultured bacterium
PelB
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 70 activity range uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 purified recombinant enzyme, 2 h, 64.9% activity remaining, half-life is 360 min uncultured bacterium
50
-
 purified recombinant enzyme, half-life is 88 min uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
750
-
 Pectin 50-70% methylated substrate, pH 9.8, 45°C, recombinant enzyme uncultured bacterium
1882
-
 Pectin over 80% methylated substrate, pH 9.8, 45°C, recombinant enzyme uncultured bacterium
2295
-
 polygalacturonic acid 20-34% methylated substrate, pH 9.8, 45°C, recombinant enzyme uncultured bacterium
6092
-
 polygalacturonic acid unmethylated substrate, pH 9.8, 45°C, recombinant enzyme uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 optimum 1, about 70% of maximal activity uncultured bacterium
9.8
-
 optimum 2, maximal activity uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
4 12 activity range uncultured bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10.4 purified recombinant enzyme, about 50% activity within this range uncultured bacterium