Any feedback?
Literature summary for 4.2.2.2 extracted from
- The catalytic mechanism and unique low pH optimum of Caldicellulosiruptor bescii family 3 pectate lyase (2015), Acta Crystallogr. Sect. D, 71, 1946-1954 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structures of seven active-site mutants, five of them in complex with intact trigalacturonic acid | Caldicellulosiruptor bescii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D107N | complete loss of activity, crystallization data | Caldicellulosiruptor bescii |
| E84A | complete loss of activity | Caldicellulosiruptor bescii |
| K108A | complete loss of activity, crystallization data | Caldicellulosiruptor bescii |
| K108A/D107N | crystallization data | Caldicellulosiruptor bescii |
| K108A/E39Q | crystallization data | Caldicellulosiruptor bescii |
| K108A/Q111A | crystallization data | Caldicellulosiruptor bescii |
| K108A/Q111N | crystallization data | Caldicellulosiruptor bescii |
| K108A/R133A | crystallization data | Caldicellulosiruptor bescii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor bescii | B9MKT4 | - |
- |
| Caldicellulosiruptor bescii DSM 6725 | B9MKT4 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | reaction mechanism can be explained by an antiperiplanar trans-elimination reaction, in which Lys108 abstracts a proton from the C5 atom. An acidified water molecule completes the anti beta-elimination reaction by protonating the O4 atom of the substrate. Both the C5 hydrogen and C4 hydroxyl groups of the substrate must be orientated in axial configurations | Caldicellulosiruptor bescii | ? | - |
? |  |
| additional information | reaction mechanism can be explained by an antiperiplanar trans-elimination reaction, in which Lys108 abstracts a proton from the C5 atom. An acidified water molecule completes the anti beta-elimination reaction by protonating the O4 atom of the substrate. Both the C5 hydrogen and C4 hydroxyl groups of the substrate must be orientated in axial configurations | Caldicellulosiruptor bescii DSM 6725 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Athe_1854 | locus name | Caldicellulosiruptor bescii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Caldicellulosiruptor bescii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
more than 10% of maximum activity | Caldicellulosiruptor bescii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
