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Literature summary for 4.2.2.2 extracted from

  • Sasaki, M.; Koide, O.; Kobayashi, T.; Usami, R.; Horikoshi, K.
    A pectate lyase from a deep subseafloor Georgenia muralis with unusual molecular characteristics (2015), Extremophiles, 19, 119-125 .
    View publication on PubMed
Search for more literature for 4.2.2.2

Inhibitors

Inhibitors Comment Organism Structure
additional information not significantly inhibitory: 5 mM dithiothreitol, 2-mercaptoethanol, N-ethylmaleimide, monoiodo acetate, EDTA, and EGTA and 0.1 mM p-chloromercuribenzoate Georgenia muralis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Georgenia muralis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration Georgenia muralis

Organism

Organism UniProt Comment Textmining
Georgenia muralis
-
-
-
Georgenia muralis JAM 3H7-3
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Georgenia muralis

Purification (Commentary)

Purification (Comment) Organism
purification by anion exchange chromatography, followed by heat treatment of the enzyme solution at 60°C for 30 min, and a gel filtration Georgenia muralis

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Georgenia muralis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
124
-
 pH 10, 50°C Georgenia muralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information best substrates are pectates with a degree of esterification of 14%, and relative degradation rates for pectic substrates with degree of esterification between 5 and 75% are more than 60% Georgenia muralis ?
-
 ?
additional information best substrates are pectates with a degree of esterification of 14%, and relative degradation rates for pectic substrates with degree of esterification between 5 and 75% are more than 60% Georgenia muralis JAM 3H7-3 ?
-
 ?
polygalacturonate
-
 Georgenia muralis unsaturated oligogalacturonides
-
 ?
polygalacturonate
-
 Georgenia muralis JAM 3H7-3 unsaturated oligogalacturonides
-
 ?

Subunits

Subunits Comment Organism
? x * 23000 plus x * 23500, SDS-PAGE Georgenia muralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Georgenia muralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 15 min, 60% residual activity Georgenia muralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.4
-
-
 Georgenia muralis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 11 40°C, stable Georgenia muralis