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Literature summary for 4.2.2.2 extracted from
- Characterization and overproduction of a thermo-alkaline pectate lyase from alkaliphilic Bacillus licheniformis with potential in ramie degumming (2017), Process Biochem., 54, 49-58 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | significant ramie (Boehmeria nivea) fiber weight loss (21.5%) is obtained following enzyme treatment and combined enzyme-chemical treatment (29.3%). The productivity may reach 48.3 U ml/h under high-cell-density cultivation for 30 h in 1l fed-batch fermenter, using Escherichia coli as host | Bacillus licheniformis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, no residual activity | Bacillus licheniformis |  |
| Fe2+ | 5 mM, 46% of initial activity | Bacillus licheniformis | |
| Fe3+ | 5 mM, 36% of initial activity | Bacillus licheniformis | |
| Hg2+ | 5 mM, 40% of initial activity | Bacillus licheniformis | |
| SDS | 0.1%, 3% of initial activity | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Polygalacturonate | Km value 0.38 mg/ml, pH 10.5, 70°C | Bacillus licheniformis | |
| additional information | - |
apple pectin | Km value 0.42 mg/ml, pH 10.5, 70°C | Bacillus licheniformis | |
| additional information | - |
citrus pectin | Km value 0.45 mg/ml, pH 10.5, 70°C | Bacillus licheniformis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | presence of Ca2+ enhances PelA stability in NaOH-glycine buffer, pH 9. About 60% activity is retained under incubation at 65°C with 0.3 mM CaCl2 | Bacillus licheniformis | |
| Co2+ | 5 mM, 138% of initial activity | Bacillus licheniformis | |
| Cu2+ | 5 mM, 122% of initial activity | Bacillus licheniformis | |
| Mg2+ | 5 mM, 133% of initial activity | Bacillus licheniformis | |
| Mn2+ | 5 mM, 116% of initial activity | Bacillus licheniformis | |
| Ni2+ | 5 mM, 120% of initial activity | Bacillus licheniformis | |
| Zn2+ | 5 mM, 145% of initial activity | Bacillus licheniformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | A0A1B1LZ58 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 153 | - |
substrate pectin, 55-70% methylated, pH 10.5, 70°C | Bacillus licheniformis |
| 157 | - |
substrate pectin, 20-34% methylated, pH 10.5, 70°C | Bacillus licheniformis |
| 211 | - |
substrate citrus pectin, pH 10.5, 70°C | Bacillus licheniformis |
| 232 | - |
substrate apple pectin, pH 10.5, 70°C | Bacillus licheniformis |
| 320 | - |
substrate polygalacturonate, pH 10.5, 70°C | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| apple pectin | - |
Bacillus licheniformis | unsaturated digalacturonic acid + unsaturated trigalacturonic acid | - |
? | |
| citrus pectin | - |
Bacillus licheniformis | unsaturated digalacturonic acid + unsaturated trigalacturonic acid | - |
? | |
| methylated pectin | - |
Bacillus licheniformis | ? | - |
? | |
| additional information | no substrates: carboxymethylcellulose, xylan, glucan, and locust bean gum | Bacillus licheniformis | ? | - |
? | |
| polygalacturonate | - |
Bacillus licheniformis | unsaturated digalacturonic acid + unsaturated trigalacturonic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PelA | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 70 | more than 80% of the original enzyme activity is retained in Tris-HCl buffers with pH 7 and 8, after incubation for 30 min | Bacillus licheniformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 142.3 | - |
citrus pectin | pH 10.5, 70°C | Bacillus licheniformis | |
| 166.5 | - |
apple pectin | pH 10.5, 70°C | Bacillus licheniformis | |
| 193.8 | - |
Polygalacturonate | pH 10.5, 70°C | Bacillus licheniformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
glycine-NaOH buffer | Bacillus licheniformis |
| 11 | - |
KCl-NaOH buffer | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | 4 h, 30°C, more than 80% resdidual activity | Bacillus licheniformis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
citrus pectin | kcat/Km value 316 ml/mg/s, pH 10.5, 70°C | Bacillus licheniformis | |
| additional information | - |
apple pectin | kcat/Km value 396 ml/mg/s, pH 10.5, 70°C | Bacillus licheniformis | |
| additional information | - |
Polygalacturonate | kcat/Km value 510 ml/mg/s, pH 10.5, 70°C | Bacillus licheniformis | |
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