Literature summary for 4.2.3.1 extracted from

Flavin, M.; Slaughter, C.
Purification and properties of threonine synthetase of Neurospora (1960), J. Biol. Chem., 235, 1103-1108.

Search for more literature for 4.2.3.1

Inhibitors

Inhibitors	Comment	Organism	Structure
KCN	22% inhibition at 1 mM	Neurospora crassa
L-cysteine	85% inhibition at 35 mM	Neurospora crassa
NH2OH	65% inhibition at 1 mM	Neurospora crassa
phosphate	40% inhibition at 50 mM	Neurospora crassa
phospho-threonine	35% inhibition at 10 mM	Neurospora crassa

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Neurospora crassa

Reaction

Reaction	Comment	Organism	Reaction ID
O-phospho-L-homoserine + H2O = L-threonine + phosphate	reaction proceeds via phospate removal and isomerization from primary to secondary alcohol	Neurospora crassa	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.73	-	-	Neurospora crassa

Storage Stability

Storage Stability	Organism
-15°C, glycylglycine, several months	Neurospora crassa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-phospho-L-homoserine + H2O	-	Neurospora crassa	L-threonine + phosphate	-	ir

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	enzyme assay at	Neurospora crassa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	enzyme assay at	Neurospora crassa

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.2	-	inactivation and precipitation below	Neurospora crassa

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	required cofactor	Neurospora crassa

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Release 2023.1 (January 2023)