Literature summary for 4.2.3.144 extracted from

Falara, V.; Alba, J.M.; Kant, M.R.; Schuurink, R.C.; Pichersky, E.
Geranyllinalool synthases in Solanaceae and other angiosperms constitute an ancient branch of diterpene synthases that are involved in the synthesis of defensive compounds (2014), Plant Physiol., 166, 428-441.

Search for more literature for 4.2.3.144

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0187	-	geranylgeranyl diphosphate	pH 7.0, temperature not specified in the publication	Solanum lycopersicum
0.0315	-	geranylgeranyl diphosphate	pH 7.0, temperature not specified in the publication	Nicotiana attenuata

Organism

Organism	UniProt	Comment	Textmining
Nicotiana attenuata	KJ755868	-	-
Solanum lycopersicum	KJ755870	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	constitutive expression	Nicotiana attenuata	-
leaf	constitutive expression	Nicotiana attenuata	-
leaf	not expressed under normal growth but induced by alamethicin and methyl jasmonate treatments	Solanum lycopersicum	-
additional information	enzyme is not expressed in any tissue under normal growth	Solanum lycopersicum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate + H2O	-	Nicotiana attenuata	(E,E)-geranyllinalool + diphosphate	-	?
geranylgeranyl diphosphate + H2O	enzyme is 55fold more efficient with geranylgeranyl diphosphate than with farnesyl diphosphate. Reaction with farnesyl diphosphate yields nerolidol	Solanum lycopersicum	(E,E)-geranyllinalool + diphosphate	single major product	?

Synonyms

Synonyms	Comment	Organism
GLS	-	Solanum lycopersicum
GLS	-	Nicotiana attenuata

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.85	-	geranylgeranyl diphosphate	pH 7.0, temperature not specified in the publication	Solanum lycopersicum
7.53	-	geranylgeranyl diphosphate	pH 7.0, temperature not specified in the publication	Nicotiana attenuata

Expression

Organism	Comment	Expression
Solanum lycopersicum	enzyme is not expressed in any tissue under normal growth but is induced in leaves by alamethicin and methyl jasmonate treatments	up
Nicotiana attenuata	in leaves, expression is induced by methyl jasmonate	up

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.24	-	geranylgeranyl diphosphate	pH 7.0, temperature not specified in the publication	Nicotiana attenuata
0.37	-	geranylgeranyl diphosphate	pH 7.0, temperature not specified in the publication	Solanum lycopersicum

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Release 2023.1 (January 2023)