Any feedback?
Literature summary for 4.2.3.144 extracted from
- Identification, functional characterization, and evolution of terpene synthases from a basal dicot (2015), Plant Physiol., 169, 1683-1697 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene LnTPS3, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) from expression vector pEXP5-CT/TOPO TA | Laurus nobilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0305 | - |
geranylgeranyl diphosphate | recombinant enzyme, pH 7.0, 30°C | Laurus nobilis |  |
| 0.0434 | - |
geranylgeranyl diphosphate | recombinant enzyme, pH 7.0, 30°C | Laurus nobilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate + H2O | Laurus nobilis | - |
(6E,10E)-geranyllinalool + diphosphate | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Methanol | 50% v/v inactivates the enzyme | Laurus nobilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Laurus nobilis | A0A0H4U599 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) LnTPS3 does not bind to the nickel-agarose column, and is instead partially purified by anion exchange chromatography and desalting gel filtration to about 5% purity | Laurus nobilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Laurus nobilis | - |
| fruit | - |
Laurus nobilis | - |
| leaf | - |
Laurus nobilis | - |
| additional information | traces of geranylgeranyl diphosphate in all tissues, and small amounts of the C16-homoterpene 4,8,12-trimethyltridacan-1,3,7,11-tetraene (TMTT), which is derived from geranyllinalool | Laurus nobilis | - |
| root | - |
Laurus nobilis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | - |
Laurus nobilis | trans-nerolidol + diphosphate | - |
? | |
| geranylgeranyl diphosphate + H2O | - |
Laurus nobilis | (6E,10E)-geranyllinalool + diphosphate | - |
? | |
| additional information | the partially purified LnTPS3 protein catalyzes the formation of geranyllinalool from geranylgeranyl diphosphate (GGPP) and trans-nerolidol from eeFPP, GC-MS analysis of reaction products. In absence of functional LnTPS3, no trans-geranyllinalool and trans-nerolidol synthase activities are detected. No activity with geranyl diphosphate | Laurus nobilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 97700, about, sequence calculation | Laurus nobilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GLS | - |
Laurus nobilis |
| LnTPS3 | - |
Laurus nobilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Laurus nobilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Laurus nobilis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | geranyllinalool synthase activity is the likely ancestral function in angiosperms of genes belonging to an ancient TPS-e/f subclade that diverged from the kaurene synthase gene lineages before the split of angiosperms and gymnosperms. The geranyllinalool synthase subclade diverged from the rest of the TPS genes in clade e/f before the split of the gymnosperm and angiosperm lineages, enzymes encoded by genes in this subclade have only been characterized from eudicot species. Laurus nobilis also has a gene belonging to the geranyllinalool synthase subclade of TPS clade e/f and the enzyme encoded by this gene is an active geranyllinalool synthase. Geranyllinalool might have been one of the first specialized metabolites to have evolved from early stages in the evolution of the TPS gene family in plants. Phylogenetic analysis, unrooted tree | Laurus nobilis |
| additional information | volatile profiles in leaves, flowers, fruits, and roots in male and female Laurus nobilis plants, overview. The biggest gender differences are observed in the flowers, where female flowers have predominantly monoterpenes as well as eugenol and methyleugenol, whereas male flowers contain mostly sesquiterpenes and benzaldehyde | Laurus nobilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
