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Literature summary for 4.2.3.146 extracted from

  • Görner, C.; Häuslein, I.; Schrepfer, P.; Eisenreich, W.; Brück, T.
    Targeted engineering of cyclooctat-9-en-7-ol synthase A stereospecific access to two new non-natural fusicoccane-type diterpenes (2013), ChemCatChem, 5, 3289-3298 .
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces melanosporofaciens

Protein Variants

Protein Variants Comment Organism
F107A mutation results in the formation of a monocyclic cembrene, which structurally bears no resemblance to the parent compound cyclooctat-9-en-7-ol Streptomyces melanosporofaciens
F107G the mutant enzyme produces cembrene A as single cyclization product Streptomyces melanosporofaciens
F107Y the mutant enzyme produces a product mixture Streptomyces melanosporofaciens
F149H the mutant enzyme produces a single cyclization product Streptomyces melanosporofaciens
F149L cyclisation of geranylgeranyl diphosphate by the the mutant enzyme results in the formation of the non-natural fusicoccane macrocycle cyclooctat-7-en-3-ol Streptomyces melanosporofaciens
F149V the mutant enzyme produces a single cyclization product Streptomyces melanosporofaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + H2O Streptomyces melanosporofaciens the enzyme is involved in biosynthesis of cyclooctatin cyclooctat-9-en-7-ol + diphosphate
-
 ?
geranylgeranyl diphosphate + H2O Streptomyces melanosporofaciens MI614-43F2 the enzyme is involved in biosynthesis of cyclooctatin cyclooctat-9-en-7-ol + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces melanosporofaciens C9K1X5
-
-
Streptomyces melanosporofaciens MI614-43F2 C9K1X5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + H2O
-
 Streptomyces melanosporofaciens cyclooctat-9-en-7-ol + diphosphate
-
 ?
geranylgeranyl diphosphate + H2O the enzyme is involved in biosynthesis of cyclooctatin Streptomyces melanosporofaciens cyclooctat-9-en-7-ol + diphosphate
-
 ?
geranylgeranyl diphosphate + H2O
-
 Streptomyces melanosporofaciens MI614-43F2 cyclooctat-9-en-7-ol + diphosphate
-
 ?
geranylgeranyl diphosphate + H2O the enzyme is involved in biosynthesis of cyclooctatin Streptomyces melanosporofaciens MI614-43F2 cyclooctat-9-en-7-ol + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
CotB2
-
 Streptomyces melanosporofaciens

General Information

General Information Comment Organism
metabolism the enzyme is involved in biosynthesis of cyclooctatin Streptomyces melanosporofaciens