Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0937 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Streptomyces sp. |
95000 | - |
gel filtration | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Streptomyces sp. | - |
cembrene C + diphosphate | cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene | ? | |
geranylgeranyl diphosphate | Streptomyces sp. SANK 60404 | - |
cembrene C + diphosphate | cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | M1V9Q0 | - |
- |
Streptomyces sp. SANK 60404 | M1V9Q0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Streptomyces sp. | cembrene C + diphosphate | cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene | ? | |
geranylgeranyl diphosphate | the bifunctional enzyme also produces (R)-nephthenol. See EC 4.2.3.149, nephthenol synthase | Streptomyces sp. | cembrene C + diphosphate | cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene | ? | |
geranylgeranyl diphosphate | - |
Streptomyces sp. SANK 60404 | cembrene C + diphosphate | cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene | ? | |
geranylgeranyl diphosphate | the bifunctional enzyme also produces (R)-nephthenol. See EC 4.2.3.149, nephthenol synthase | Streptomyces sp. SANK 60404 | cembrene C + diphosphate | cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene | ? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42000, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
DtcycA | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay | Streptomyces sp. |