Cloned (Comment) | Organism |
---|---|
phylogenetic tree, recombinant expression of wild-type and mutant enzymes in Escherichia coli | Allokutzneria albata |
Protein Variants | Comment | Organism |
---|---|---|
D212E | site-directed mutagenesis, the mutation results in strongly decreased activity with Mg2+ and Mn2+ | Allokutzneria albata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Allokutzneria albata | |
Mn2+ | activates | Allokutzneria albata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Allokutzneria albata | - |
(S)-(+)-cembrene A + diphosphate | - |
? | |
geranylgeranyl diphosphate | Allokutzneria albata DSM 44149 | - |
(S)-(+)-cembrene A + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Allokutzneria albata | - |
isolated from tropical soil | - |
Allokutzneria albata DSM 44149 | - |
isolated from tropical soil | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
geranylgeranyl diphosphate = (R)-cembrene A + diphosphate | the cyclization mechanism from geranylgeranyl diphosphate to cembrene A requires a 1,14-cyclization to the ent-cembranyl cation and deprotonation of one of the geminal methyl groups, reaction mechanism, overview | Allokutzneria albata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enzyme seems to be not expressed in laboratory cultures | Allokutzneria albata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Allokutzneria albata | (S)-(+)-cembrene A + diphosphate | - |
? | |
geranylgeranyl diphosphate | - |
Allokutzneria albata DSM 44149 | (S)-(+)-cembrene A + diphosphate | - |
? | |
additional information | product identification by NMR spectroscopic analysis. Development of a method that uses the stereoselectively deuterated precursors (S)- and (R)-(1-13C,1-2H)farnesyl diphosphate and (S)- and (R)-(1-13C,1-2H)geranyl diphosphate to determine the absolute configurations of terpenes | Allokutzneria albata | ? | - |
? | |
additional information | product identification by NMR spectroscopic analysis. Development of a method that uses the stereoselectively deuterated precursors (S)- and (R)-(1-13C,1-2H)farnesyl diphosphate and (S)- and (R)-(1-13C,1-2H)geranyl diphosphate to determine the absolute configurations of terpenes | Allokutzneria albata DSM 44149 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAS | - |
Allokutzneria albata |