Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.158 extracted from

  • Rabe, P.; Rinkel, J.; Dolja, E.; Schmitz, T.; Nubbemeyer, B.; Luu, T.H.; Dickschat, J.S.
    Mechanistic investigations of two bacterial diterpene cyclases: spiroviolene synthase and tsukubadiene synthase (2017), Angew. Chem. Int. Ed. Engl., 56, 2776-2779.
    View publication on PubMed
Search for more literature for 4.2.3.158

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the expression vector pYEExpress Streptomyces violens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Streptomyces violens
-
 (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate Streptomyces violens NRRL ISP-5597
-
 (-)-spiroviolene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Allokutzneria albata A0A1G9TH01
-
-
Allokutzneria albata NRRL B-24461 A0A1G9TH01
-
-
Streptomyces ambofaciens
-
 UniParc: UPI00069D7986
-
Streptomyces ambofaciens ATCC 23877
-
 UniParc: UPI00069D7986
-
Streptomyces ochraceiscleroticus
-
 UniParc: UPI0004C5E0B8
-
Streptomyces ochraceiscleroticus NRRL ISP-5594
-
 UniParc: UPI0004C5E0B8
-
Streptomyces sclerotialus
-
 UniParc: UPI0004C53AD1
-
Streptomyces sclerotialus NRRL ISP-5269
-
 UniParc: UPI0004C53AD1
-
Streptomyces violens
-
 UniParc: UPI0004BE9F6E
-
Streptomyces violens NRRL ISP-5597
-
 UniParc: UPI0004BE9F6E
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Streptomyces violens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Streptomyces violens (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (-)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene] Allokutzneria albata (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (-)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene] Streptomyces ochraceiscleroticus (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (-)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene] Streptomyces sclerotialus (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (-)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene]. For the geminal methyl groups C16 and C17 of geranylgeranyl diphosphate the enzyme shows a strictly controlled stereochemical course, as observed for most terpene cyclases. No activity with geranyl diphosphate and farnesyl diphosphate Streptomyces violens (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (–)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene] Streptomyces ambofaciens (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (-)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene] Streptomyces ochraceiscleroticus NRRL ISP-5594 (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate
-
 Streptomyces violens NRRL ISP-5597 (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (-)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene]. For the geminal methyl groups C16 and C17 of geranylgeranyl diphosphate the enzyme shows a strictly controlled stereochemical course, as observed for most terpene cyclases. No activity with geranyl diphosphate and farnesyl diphosphate Streptomyces violens NRRL ISP-5597 (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (–)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene] Streptomyces ambofaciens ATCC 23877 (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (-)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene] Allokutzneria albata NRRL B-24461 (-)-spiroviolene + diphosphate
-
 ?
geranylgeranyl diphosphate (-)-spiroviolene i.e. (2R,3a'S,3b'R,5R,6a'R)-2,4',4',5,6a'-pentamethyl-2',3',3a',3b',4',5',6',6a'-octahydrospiro[cyclopentane-1,1'-cyclopenta[a]pentalene] Streptomyces sclerotialus NRRL ISP-5269 (-)-spiroviolene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
SvS
-
 Streptomyces ambofaciens
SvS
-
 Allokutzneria albata
SvS
-
 Streptomyces violens
SvS
-
 Streptomyces ochraceiscleroticus
SvS
-
 Streptomyces sclerotialus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Streptomyces violens