Literature summary for 4.2.3.159 extracted from

Rabe, P.; Rinkel, J.; Dolja, E.; Schmitz, T.; Nubbemeyer, B.; Luu, T.H.; Dickschat, J.S.
Mechanistic investigations of two bacterial diterpene cyclases: spiroviolene synthase and tsukubadiene synthase (2017), Angew. Chem. Int. Ed. Engl., 56, 2776-2779.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces tsukubensis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces tsukubensis	I2N045	-	-
Streptomyces tsukubensis NRRL 18488	I2N045	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces tsukubensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate	tsukubadiene i.e. (1S,3aS,5Z,7aS,10aR,11Z)-1,5,8,8,10a-pentamethyl-2,3,3a,4,7,7a,8,9,10,10a-decahydro-1H-dicyclopenta[a,d][9]annulene. No activity with geranyl diphosphate and farnesyl diphosphate. Cyclization mechanism: The first elementary reaction proceeds by 1,11-Si-cyclization of geranylgeranyl diphosphate. In this step, the same absolute configuration at C11 is installed and is not changed during the further process	Streptomyces tsukubensis	tsukubadiene + diphosphate	-	?
geranylgeranyl diphosphate	tsukubadiene i.e. (1S,3aS,5Z,7aS,10aR,11Z)-1,5,8,8,10a-pentamethyl-2,3,3a,4,7,7a,8,9,10,10a-decahydro-1H-dicyclopenta[a,d][9]annulene. No activity with geranyl diphosphate and farnesyl diphosphate. Cyclization mechanism: The first elementary reaction proceeds by 1,11-Si-cyclization of geranylgeranyl diphosphate. In this step, the same absolute configuration at C11 is installed and is not changed during the further process	Streptomyces tsukubensis NRRL 18488	tsukubadiene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
TdS	-	Streptomyces tsukubensis

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Release 2023.1 (January 2023)