Activating Compound | Comment | Organism | Structure |
---|---|---|---|
taxol | exogenous Taxol (paclitaxel) causes an considerable increase in taxadiene synthase activity, the expression level peaks at day 4 with a rapid decrease at day 8, reaching a second, lower, peak at day 12. By day 16, ts mRNA is only detectable in the untreated control at a very low level. The concentration of taxol that most effectively induces ts mRNA expression is 50 mg/l compared to the control, the transcript levels are 2.5-, 3.1- and 3.3fold higher at 4, 8 and 12 days of culture. | Taxus baccata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM is included in assay medium | Taxus baccata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Taxus baccata | the first committed step toward taxanes and taxol biosynthesis involves taxadiene synthase which promotes the cyclization of geranylgeranyl diphosphate leading to the taxane skeleton | taxa-4,11-diene + diphosphate | the product of the biosynthetic step is not a limiting substrate in taxol formation | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus baccata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Taxus baccata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Taxus baccata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
a relatively high level of activity is observed at day 4, when it is 2.8-, 6.8-, 3.2- and 1.7fold higher, respectively, in the cultures treated with 25, 50, 100 and 200 mg/l taxol than in the untreated control (6.64 pkat/mg of protein). Enzyme activity then decreases markedly from days 4 to 12 and thereafter is measurable only at a very low level until day 20. | Taxus baccata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | the first committed step toward taxanes and taxol biosynthesis involves taxadiene synthase which promotes the cyclization of geranylgeranyl diphosphate leading to the taxane skeleton | Taxus baccata | taxa-4,11-diene + diphosphate | the product of the biosynthetic step is not a limiting substrate in taxol formation | ? |
Synonyms | Comment | Organism |
---|---|---|
taxadiene synthase | - |
Taxus baccata |
TXS | - |
Taxus baccata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
assay at | Taxus baccata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Taxus baccata |