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Literature summary for 4.2.3.19 extracted from

  • Fall, R.R.; West, C.A.
    Purification and properties of kaurene synthetase from Fusarium moniliforme (1971), J. Biol. Chem., 246, 6913-6928.
    View publication on PubMed
Search for more literature for 4.2.3.19

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Fusarium verticillioides
cysteine
-
 Fusarium verticillioides
dithiothreitol stimulates activity in presence of EDTA Fusarium verticillioides

General Stability

General Stability Organism
sensitive on freezing and thawing Fusarium verticillioides

Inhibitors

Inhibitors Comment Organism Structure
2-(N,N-dimethyl-N-octylammonium bromide)-p-methan-1-ol Q-64 Fusarium verticillioides
copalyl diphosphate slight substrate inhibition at concentrations above 0.0007 mM Fusarium verticillioides
Cu2+
-
 Fusarium verticillioides
deoxycholate
-
 Fusarium verticillioides
EDTA
-
 Fusarium verticillioides
Hg2+
-
 Fusarium verticillioides
iodacetamide
-
 Fusarium verticillioides
N,N-dimethylaminoethyl 2,2-diphenylphenylether SKF 3301A Fusarium verticillioides
N-ethylmaleimide
-
 Fusarium verticillioides
p-hydroxymercuribenzoate
-
 Fusarium verticillioides
Phosphone D
-
 Fusarium verticillioides
Phosphone S
-
 Fusarium verticillioides
sulfhydryl groups
-
 Fusarium verticillioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 copalyl diphosphate
-
 Fusarium verticillioides

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Fusarium verticillioides
Co2+ can partially replace Mg2+ Fusarium verticillioides
Mg2+ required, optimum concentration 5 mM Fusarium verticillioides
Mn2+ can partially replace Mg2+ Fusarium verticillioides
Ni2+ can partially replace Mg2+ Fusarium verticillioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
430000
-
 sucrose density gradient centrifugation, crude enzyme Fusarium verticillioides
450000
-
 sucrose density gradient centrifugation, purified enzyme Fusarium verticillioides
490000
-
 gel filtration Fusarium verticillioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Fusarium verticillioides involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium verticillioides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, copurified with ent-kaurene synthase A (EC 5.5.1.13) activity Fusarium verticillioides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.123
-
 purified enzyme Fusarium verticillioides

Storage Stability

Storage Stability Organism
-20°C, 25% glycerol, several months, no loss of activity for purified enzyme Fusarium verticillioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
copalyl diphosphate
-
 Fusarium verticillioides sandaracopimaradiene + diphosphate
-
 ?
ent-copalyl diphosphate
-
 Fusarium verticillioides ent-kaurene + diphosphate
-
 ?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Fusarium verticillioides ent-kaurene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Fusarium verticillioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 TES or HEPES buffer Fusarium verticillioides
7.5
-
 phosphate buffer Fusarium verticillioides