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Literature summary for 4.2.3.19 extracted from

  • Frost, R.G.; West, C.A.
    Properties of kaurene synthetase from Marah macrocarpus (1977), Plant Physiol., 59, 22-29.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.19

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 0.001-10 mM, eliminated in presence of 0.1 mM EDTA Marah macrocarpa

Inhibitors

Inhibitors Comment Organism Structure
2'-isopropyl-4'(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate Amo-1618; strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity Marah macrocarpa
2-(N,N-dimethyl-N-octylammonium bromide)-p-methan-1-ol strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity Marah macrocarpa
copalyl diphosphate substrate inhibition above 0.0002 mM, might be an effect of contaminants in substrate preparations Marah macrocarpa
EDTA
-
 Marah macrocarpa
N,N-dimethylaminoethyl 2,2-diphenylphenylether strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity Marah macrocarpa
N-ethylmaleimide
-
 Marah macrocarpa
p-hydroxymercuribenzoate
-
 Marah macrocarpa
Phosphone D strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity Marah macrocarpa
Phosphone S strong inhibition of EC 5.5.1.13 activity, very weak inhibition of EC 4.2.3.19 activity Marah macrocarpa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 copalyl diphosphate
-
 Marah macrocarpa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Marah macrocarpa
Co2+ can partially replace Mg2+ Marah macrocarpa
Mg2+
-
 Marah macrocarpa
Mn2+ can partially replace Mg2+ Marah macrocarpa
Ni2+ very low activity Marah macrocarpa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration, molecular weight less than 45000 Da Marah macrocarpa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Marah macrocarpa involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Marah macrocarpa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Marah macrocarpa

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Marah macrocarpa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
 partially purified enzyme Marah macrocarpa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
copalyl diphosphate
-
 Marah macrocarpa sandaracopimaradiene + diphosphate
-
 ?
ent-copalyl diphosphate
-
 Marah macrocarpa ent-kaurene + diphosphate
-
 ?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Marah macrocarpa ent-kaurene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Marah macrocarpa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 7.2 TES buffer Marah macrocarpa
6.9
-
 imidazole buffer Marah macrocarpa
7.2 8.2 Tris buffer Marah macrocarpa