Literature summary for 4.2.3.194 extracted from

Kwon, M.; Cochrane, S.A.; Vederas, J.C.; Ro, D.K.
Molecular cloning and characterization of drimenol synthase from valerian plant (Valeriana officinalis) (2014), FEBS Lett., 588, 4597-4603 .

Search for more literature for 4.2.3.194

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae	Valeriana officinalis

Protein Variants

Protein Variants	Comment	Organism
D307A	mutation of the first Asp residue of the DDxxD motif, known to ionize the substrate in concert with Mg2+. Mutant is unable to ionize substrate farnesyl diphosphate	Valeriana officinalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0096	-	(2E,6E)-farnesyl diphosphate	pH 7.5, 30°C	Valeriana officinalis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	assay in presence of	Valeriana officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + H2O	Valeriana officinalis	-	(-)-drimenol + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Valeriana officinalis	L0HQE3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + H2O	-	Valeriana officinalis	(-)-drimenol + diphosphate	-	?
additional information	possible intermediates, farnesol and drimenyl diphosphate, cannot be converted to drimenol, suggesting that the intermediate remains tightly bound during catalysis. Enzyme likely uses protonation-initiated cyclization	Valeriana officinalis	?	-	?

Synonyms

Synonyms	Comment	Organism
sesquiterpene synthase 3	-	Valeriana officinalis
TPS3	-	Valeriana officinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.025	-	(2E,6E)-farnesyl diphosphate	pH 7.5, 30°C	Valeriana officinalis

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Release 2023.1 (January 2023)