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Literature summary for 4.2.3.27 extracted from
- Biochemical characterization of an isoprene synthase from Campylopus introflexus (heath star moss) (2015), Plant Physiol. Biochem., 94, 209-215 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | pre-incubation of the partially-purified enzyme with 5 mM N-ethylmaleimide results in 66% inhibition of activity | Campylopus introflexus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
dimethylallyl diphosphate | at pH 8.6 and 40°C | Campylopus introflexus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, 50-60% activity at 15 mM, the enzyme utilizes Mn2+ preferentially over Mg2+ | Campylopus introflexus | |
| Mn2+ | required, 100% activity at 15 mM, the enzyme utilizes Mn2+ preferentially over Mg2+ | Campylopus introflexus | |
| additional information | not activated by Zn2+ or Ca2+ | Campylopus introflexus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | Campylopus introflexus | - |
isoprene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylopus introflexus | - |
heath star moss | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE column chromatography, and Superdex 200 gel filtration | Campylopus introflexus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shoot | - |
Campylopus introflexus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | - |
Campylopus introflexus | isoprene + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ISPS | - |
Campylopus introflexus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Campylopus introflexus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Campylopus introflexus |
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Release 2023.1 (January 2023)
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