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Literature summary for 4.2.99.18 extracted from
- Purification and characterization of an endonuclease specific for sites in DNA from a permanently established mouse palsmocytoma cell line (1980), Nucleic Acids Res., 8, 1575-1589.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | complete inhibition at 2 mM | Mus musculus |  |
| EDTA | complete inhibition at 2 mM | Mus musculus | |
| Sodium citrate | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000027 | - |
DNA containing apurinic site | 37°C, pH 8 | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | stimulates | Mus musculus | |
| KCl | optimal concentration: 50 mM,90% inhibition at 200 mM | Mus musculus | |
| Mg2+ | stimulates | Mus musculus | |
| Mg2+ | optimal concentration: 4-5 mM | Mus musculus | |
| Mn2+ | stimulates | Mus musculus | |
| Mn2+ | optimum: 4-5 mM | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | Mus musculus | - |
fragments of DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| MPC-11 cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA | - |
Mus musculus | fragments of DNA | - |
? | |
| DNA | specific for apurinic sites | Mus musculus | fragments of DNA | - |
? | |
| DNA | no activity with methylated or OsO4-treated DNA | Mus musculus | fragments of DNA | - |
? | |
| DNA containing apurinic site | - |
Mus musculus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.5 | pH 6.5: about 25% of activity maximum, pH 10.5: about 20% of activity maximum | Mus musculus |
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Release 2023.1 (January 2023)
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