Activating Compound | Comment | Organism | Structure |
---|---|---|---|
8-oxoguanine-DNA glycosylase | OGG1 stimulates the hydrolytic activity of AP endonuclease | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into the pET28b+ vector for expression in Escherichia BL21DE3 RIL cells | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
determined by SDS-PAGE and Western blotting | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation and affinity chromatography, using a HiTrap chelating column, charged with Ni2+, and a HiTrap heparin column | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
AP endonuclease | - |
Homo sapiens |
APE1 | - |
Homo sapiens |
apurinic/apyrimidinic endonuclease 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Homo sapiens |