Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.99.18 extracted from

  • Hecker, A.; Leulliot, N.; Gadelle, D.; Graille, M.; Justome, A.; Dorlet, P.; Brochier, C.; Quevillon-Cheruel, S.; Le Cam, E.; van Tilbeurgh, H.; Forterre, P.
    An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitro (2007), Nucleic Acids Res., 35, 6042-6051.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Escherichia coli Pyrococcus abyssi

Crystallization (Commentary)

Crystallization (Comment) Organism
structures are deposited into the Brookhaven Protein Data Bank under the accession numbers 2IVN, 2IVO and 2IVP, structure is solved to 3.0 A and further refined to 1.6 A Pyrococcus abyssi

Protein Variants

Protein Variants Comment Organism
Y127F decreased endonuclease activity, Tyr 127 is essential to the binding and stabilization of the Fe3+ ion, mutation do not affect DNA binding Pyrococcus abyssi

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Pyrococcus abyssi
EDTA
-
Pyrococcus abyssi

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+
-
Pyrococcus abyssi
Mg2+
-
Pyrococcus abyssi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36200
-
recombinant histidine-tagged Pa-Kae1 Pyrococcus abyssi

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus abyssi

Subunits

Subunits Comment Organism
monomer the purified protein is present as a monomer in solution Pyrococcus abyssi

Synonyms

Synonyms Comment Organism
AP-endonuclease
-
Pyrococcus abyssi
AP-lyase
-
Pyrococcus abyssi
apurinic endonuclease
-
Pyrococcus abyssi
Kae1 kinase-associated endopeptidase 1 exibits a class I apurinic endonuclease activity, no activity in vitro Pyrococcus abyssi
PaKae1
-
Pyrococcus abyssi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
DNA-binding assay Pyrococcus abyssi
72
-
AP-endonuclease assay Pyrococcus abyssi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
AP-endonuclease assay Pyrococcus abyssi
7.5
-
DNA-binding assay Pyrococcus abyssi