BRENDA - Enzyme Database
show all sequences of 4.2.99.20

Crystal structures of E. coli native MenH and two active site mutants

Johnston, J.M.; Jiang, M.; Guo, Z.; Baker, E.N.; PLoS ONE 8, e61325 (2013)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
native enzyme to 2.75 A resolution, together with the structures of the active site mutant proteins Tyr85Phe and Arg124Ala, both at 2.5 A resolution. The enzyme has the predicted alpha/beta hydrolase fold with its core alpha/beta domain capped by a helical lid. The active site, a long groove beneath the cap, contains a number of conserved basic residues and is found to bind exogeneous anions, modeled as sulfate and chloride, in all three crystal structures. The bound anions may mark the binding sites for anionic groups on the substrate
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P37355
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Crystallization (Commentary) (protein specific)
Crystallization
Organism
native enzyme to 2.75 A resolution, together with the structures of the active site mutant proteins Tyr85Phe and Arg124Ala, both at 2.5 A resolution. The enzyme has the predicted alpha/beta hydrolase fold with its core alpha/beta domain capped by a helical lid. The active site, a long groove beneath the cap, contains a number of conserved basic residues and is found to bind exogeneous anions, modeled as sulfate and chloride, in all three crystal structures. The bound anions may mark the binding sites for anionic groups on the substrate
Escherichia coli
Other publictions for EC 4.2.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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289
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730729
Johnston
Crystal structures of E. coli ...
Escherichia coli
PLoS ONE
8
e61325
2013
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714632
Dawson
Exploiting the high-resolution ...
Staphylococcus aureus
BMC Struct. Biol.
11
19
2011
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1
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702326
Jiang
Catalytic mechanism of SHCHC s ...
Escherichia coli
Biochemistry
48
6921-6931
2009
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1
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12
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13
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13
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13
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685225
Jiang
Identification and characteriz ...
Escherichia coli
Biochemistry
47
3426-3434
2008
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3
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685086
Jiang
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Escherichia coli K-12
Biochemistry
46
10979-10989
2007
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