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Literature summary for 4.3.1.18 extracted from
- A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae (2008), Biochem. J., 409, 399-406.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET15b vector for expression in Escherichia coli BL21DE3 cells | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 10.2%, without 10.0% | Saccharomyces cerevisiae |  |
| hydroxylamine | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 64.4%, without 23.7% | Saccharomyces cerevisiae | |
| N-ethylmaleimide | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 19.5%, without 8.7% | Saccharomyces cerevisiae | |
| phenylhydrazin | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 97.1%, without 4.2% | Saccharomyces cerevisiae | |
| Sodium cyanoborohydride | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 20.9%, without 12.1% | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
D-threonine | untreated | Saccharomyces cerevisiae | |
| 0.17 | - |
D-threonine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 0.19 | - |
D-serine | EDTA-treated | Saccharomyces cerevisiae | |
| 0.39 | - |
D-serine | untreated | Saccharomyces cerevisiae | |
| 0.48 | - |
D-serine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 0.98 | - |
beta-Chloro-D-alanine | EDTA-treated | Saccharomyces cerevisiae | |
| 1.45 | - |
beta-Chloro-D-alanine | untreated | Saccharomyces cerevisiae | |
| 2.45 | - |
beta-Cl-D-alanine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
determined by SDS-PAGE | Saccharomyces cerevisiae |
| 50060 | - |
calculated molecular mass | Saccharomyces cerevisiae |
| 118000 | - |
dimer, determined by gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-serine | Saccharomyces cerevisiae | - |
pyruvate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Ni2+-chelating column and a DEAE-TOYOPEARL column | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-chloro-D-alanine | activity of approximately 2% | Saccharomyces cerevisiae | 3-chloro-D-alanine | - |
? | |
| D-serine | - |
Saccharomyces cerevisiae | pyruvate + NH3 | - |
? | |
| D-threonine | activity of approximately 3% | Saccharomyces cerevisiae | 2-oxobutanoate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-serine ammonia-lyase | - |
Saccharomyces cerevisiae |
| D-serine dehydratase | - |
Saccharomyces cerevisiae |
| DSD | - |
Saccharomyces cerevisiae |
| DsdSC | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ammonia assay | Saccharomyces cerevisiae |
| 30 | - |
pyruvate assay | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
beta-Chloro-D-alanine | untreated | Saccharomyces cerevisiae | |
| 0.24 | - |
beta-Chloro-D-alanine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 0.34 | - |
D-threonine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 0.43 | - |
D-threonine | untreated | Saccharomyces cerevisiae | |
| 0.48 | - |
beta-Chloro-D-alanine | EDTA-treated | Saccharomyces cerevisiae | |
| 1.67 | - |
D-serine | EDTA-treated | Saccharomyces cerevisiae | |
| 12.5 | - |
D-serine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 13.1 | - |
D-serine | untreated | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
ammonia assay | Saccharomyces cerevisiae |
| 8 | - |
pyruvate assay | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | PLP | Saccharomyces cerevisiae | |
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