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Literature summary for 4.3.1.18 extracted from
- D-serine dehydratase from chicken kidney: A vertebral homologue of the cryptic enzyme from Burkholderia cepacia (2008), J. Biochem., 143, 49-57.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pCR4-TOPO vector for sequencing | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aminooxyacetate | - |
Gallus gallus |  |
| hydroxylamine | - |
Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.131 | - |
D-serine | - |
Gallus gallus | |
| 0.312 | - |
D-threonine | - |
Gallus gallus | |
| 12.3 | - |
L-serine | - |
Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
monomer 1, determined by SDS-PAGE | Gallus gallus |
| 40400 | - |
monomer 2, determined by SDS-PAGE | Gallus gallus |
| 77000 | - |
molecular weight of the native enzyme, estimated by GC-LALLS | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-serine | Gallus gallus | - |
pyruvate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
adult male White Leghorn chicken | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from chicken kidneys using a DEAE-Sepharose, a hydroxyapatite, a Mono Q HR5/5 and a TSKgel SuperSW3000 column | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | proximal tubule | Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.004 | - |
crude extract | Gallus gallus |
| 0.094 | - |
DEAE-Sepharose purified | Gallus gallus |
| 0.52 | - |
ammonium sulfate precipitation | Gallus gallus |
| 0.91 | - |
Mono Q purified | Gallus gallus |
| 1.09 | - |
Hydroxyapatite purified | Gallus gallus |
| 1.39 | - |
SuperSW3000 purified | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-serine | - |
Gallus gallus | pyruvate + NH3 | - |
? | |
| D-threonine | - |
Gallus gallus | ? | - |
? | |
| L-serine | - |
Gallus gallus | pyruvate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 40000 + 1 * 40400, SDS-PAGE | Gallus gallus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-serine ammonia-lyase | - |
Gallus gallus |
| D-serine dehydratase | - |
Gallus gallus |
| DSD | - |
Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Gallus gallus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0512 | - |
D-threonine | - |
Gallus gallus | |
| 0.197 | - |
L-serine | - |
Gallus gallus | |
| 0.811 | - |
D-serine | - |
Gallus gallus | |
| 6.08 | - |
D-serine | - |
Gallus gallus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
activity assay | Gallus gallus |
| 9 | - |
- |
Gallus gallus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 11.5 | in the range of pH 6.5-11.5 the enzyme has an activity between 50 and 100% with an maximum at pH 9 | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Gallus gallus | |
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Release 2023.1 (January 2023)
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