Crystallization (Comment) | Organism |
---|---|
- |
Citrobacter amalonaticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 70% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
5,5'-dithiobis(2-nitrobenzoate) | complete inhibition in the deamination reaction | Citrobacter amalonaticus | |
8-hydroxyquinoline | 58% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
Ag+ | complete | Citrobacter amalonaticus | |
Ca2+ | 55% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
Ca2+ | - |
Clostridium tetanomorphum | |
Cd2+ | complete inhibition in the deamination reaction with L-threo-3-methylaspartate | Citrobacter amalonaticus | |
Co2+ | 58% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
Cu2+ | 50% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
Cu2+ | - |
Clostridium tetanomorphum | |
Fe2+ | 43% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
Hg+ | complete | Citrobacter amalonaticus | |
Hg2+ | complete | Citrobacter amalonaticus | |
hydroxylamine | - |
Clostridium tetanomorphum | |
additional information | - |
Citrobacter amalonaticus | |
N-ethylmaleimide | 36% loss of activity in the deamination raction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
N-ethylmaleimide | - |
Clostridium tetanomorphum | |
o-phenanthroline | 24% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
p-chloromercuribenzoate | complete | Citrobacter amalonaticus | |
phenylhydrazine | - |
Clostridium tetanomorphum | |
Sn2+ | 34% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
Zn2+ | 30% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Clostridium tetanomorphum | |
Co2+ | activates | Citrobacter amalonaticus | |
Cs+ | required | Citrobacter amalonaticus | |
K+ | - |
Clostridium tetanomorphum | |
K+ | - |
Citrobacter amalonaticus | |
Li+ | required | Citrobacter amalonaticus | |
Mg2+ | - |
Clostridium tetanomorphum | |
Mg2+ | - |
Citrobacter amalonaticus | |
Mn2+ | activates | Clostridium tetanomorphum | |
Mn2+ | activates | Citrobacter amalonaticus | |
additional information | - |
Clostridium tetanomorphum | |
additional information | - |
Citrobacter amalonaticus | |
Na+ | required | Citrobacter amalonaticus | |
NH4+ | required | Citrobacter amalonaticus | |
Ni2+ | activates | Clostridium tetanomorphum | |
Ni2+ | activates | Citrobacter amalonaticus | |
Rb+ | required | Citrobacter amalonaticus | |
Zn2+ | activates | Clostridium tetanomorphum | |
Zn2+ | activates | Citrobacter amalonaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Citrobacter amalonaticus |
84000 | - |
gel filtration | Citrobacter amalonaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threo-3-methylaspartate | Clostridium tetanomorphum | - |
? | - |
? | |
L-threo-3-methylaspartate | Citrobacter amalonaticus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter amalonaticus | - |
YG-1002 | - |
Citrobacter amalonaticus YG-1002 | - |
YG-1002 | - |
Clostridium tetanomorphum | - |
- |
- |
Clostridium tetanomorphum | - |
NCIMB 11547 | - |
Clostridium tetanomorphum H1 / ATCC 15920 | - |
- |
- |
Clostridium tetanomorphum NCIMB 11547 | - |
NCIMB 11547 | - |
Purification (Comment) | Organism |
---|---|
- |
Citrobacter amalonaticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Clostridium tetanomorphum |
additional information | - |
- |
Citrobacter amalonaticus |
179 | - |
- |
Citrobacter amalonaticus |
282 | - |
incubation with 5 mM EDTA, pH 7.0, 4¦C, for 3 h | Citrobacter amalonaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3R)-3-ethylaspartic acid | - |
Clostridium tetanomorphum | ? | - |
? | |
(2S,3R)-3-ethylaspartic acid | - |
Citrobacter amalonaticus | ? | - |
? | |
(2S,3S)-3-ethylaspartic acid | - |
Clostridium tetanomorphum | ? | - |
? | |
(2S,3S)-3-ethylaspartic acid | - |
Citrobacter amalonaticus | ? | - |
? | |
3-chloroaspartate | - |
Clostridium tetanomorphum | chlorofumarate + NH3 | - |
? | |
3-chloroaspartate | (2R,3S)-3-chloroaspartic acid | Citrobacter amalonaticus | chlorofumarate + NH3 | - |
? | |
3-chloroaspartate | (2R,3S)-3-chloroaspartic acid | Citrobacter amalonaticus YG-1002 | chlorofumarate + NH3 | - |
? | |
3-chloroaspartate | - |
Clostridium tetanomorphum H1 / ATCC 15920 | chlorofumarate + NH3 | - |
? | |
3-chloroaspartate | - |
Clostridium tetanomorphum NCIMB 11547 | chlorofumarate + NH3 | - |
? | |
ethylfumarate + NH3 | - |
Clostridium tetanomorphum | ? | - |
? | |
ethylfumarate + NH3 | - |
Citrobacter amalonaticus | ? | - |
? | |
ethylfumarate + NH3 | - |
Citrobacter amalonaticus YG-1002 | ? | - |
? | |
ethylfumarate + NH3 | - |
Clostridium tetanomorphum H1 / ATCC 15920 | ? | - |
? | |
ethylfumarate + NH3 | - |
Clostridium tetanomorphum NCIMB 11547 | ? | - |
? | |
L-threo-3-methylaspartate | r | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | r | Citrobacter amalonaticus | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter amalonaticus | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | r | Citrobacter amalonaticus YG-1002 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter amalonaticus YG-1002 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | r | Clostridium tetanomorphum H1 / ATCC 15920 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum H1 / ATCC 15920 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | r | Clostridium tetanomorphum NCIMB 11547 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum NCIMB 11547 | mesaconate + NH3 | - |
? | |
L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | ? | - |
? | |
L-threo-3-methylaspartate | - |
Citrobacter amalonaticus | ? | - |
? | |
additional information | - |
Clostridium tetanomorphum | ? | - |
? | |
additional information | - |
Citrobacter amalonaticus | ? | - |
? | |
additional information | - |
Citrobacter amalonaticus YG-1002 | ? | - |
? | |
additional information | - |
Clostridium tetanomorphum H1 / ATCC 15920 | ? | - |
? | |
additional information | - |
Clostridium tetanomorphum NCIMB 11547 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Clostridium tetanomorphum |
dimer | 2 * 42000, SDS-PAGE | Citrobacter amalonaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
maximal activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 6% loss of activity | Citrobacter amalonaticus |
50 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 10% loss of activity | Citrobacter amalonaticus |
55 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 44% loss of activity | Citrobacter amalonaticus |
60 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methxylaspartate, 96.5% loss of activity | Citrobacter amalonaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate | Citrobacter amalonaticus |
9.7 | - |
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Citrobacter amalonaticus |
5.5 | 11 | 30¦C, 10 min, deamination reaction of L-threo-3-methylaspartate, 14% loss of activity | Citrobacter amalonaticus |