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Literature summary for 4.3.1.2 extracted from

  • Kato, Y.; Asano, Y.
    3-Methylaspartate ammonia-lyase from a facultative anaerobe, strain YG-1002 (1995), Appl. Microbiol. Biotechnol., 43, 901-907.
    View publication on PubMed
Search for more literature for 4.3.1.2

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Citrobacter amalonaticus

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 70% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus
5,5'-dithiobis(2-nitrobenzoate) complete inhibition in the deamination reaction Citrobacter amalonaticus
8-hydroxyquinoline 58% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus
Ag+ complete Citrobacter amalonaticus
Ca2+ 55% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus
Ca2+
-
 Clostridium tetanomorphum
Cd2+ complete inhibition in the deamination reaction with L-threo-3-methylaspartate Citrobacter amalonaticus
Co2+ 58% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus
Cu2+ 50% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus
Cu2+
-
 Clostridium tetanomorphum
Fe2+ 43% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus
Hg+ complete Citrobacter amalonaticus
Hg2+ complete Citrobacter amalonaticus
hydroxylamine
-
 Clostridium tetanomorphum
additional information
-
 Citrobacter amalonaticus
N-ethylmaleimide 36% loss of activity in the deamination raction of L-threo-3-methylaspartate Citrobacter amalonaticus
N-ethylmaleimide
-
 Clostridium tetanomorphum
o-phenanthroline 24% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus
p-chloromercuribenzoate complete Citrobacter amalonaticus
phenylhydrazine
-
 Clostridium tetanomorphum
Sn2+ 34% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus
Zn2+ 30% loss of activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Clostridium tetanomorphum
Co2+ activates Citrobacter amalonaticus
Cs+ required Citrobacter amalonaticus
K+
-
 Clostridium tetanomorphum
K+
-
 Citrobacter amalonaticus
Li+ required Citrobacter amalonaticus
Mg2+
-
 Clostridium tetanomorphum
Mg2+
-
 Citrobacter amalonaticus
Mn2+ activates Clostridium tetanomorphum
Mn2+ activates Citrobacter amalonaticus
additional information
-
 Clostridium tetanomorphum
additional information
-
 Citrobacter amalonaticus
Na+ required Citrobacter amalonaticus
NH4+ required Citrobacter amalonaticus
Ni2+ activates Clostridium tetanomorphum
Ni2+ activates Citrobacter amalonaticus
Rb+ required Citrobacter amalonaticus
Zn2+ activates Clostridium tetanomorphum
Zn2+ activates Citrobacter amalonaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Citrobacter amalonaticus
84000
-
 gel filtration Citrobacter amalonaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threo-3-methylaspartate Clostridium tetanomorphum
-
 ?
-
 ?
L-threo-3-methylaspartate Citrobacter amalonaticus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Citrobacter amalonaticus
-
 YG-1002
-
Citrobacter amalonaticus YG-1002
-
 YG-1002
-
Clostridium tetanomorphum
-
-
-
Clostridium tetanomorphum
-
 NCIMB 11547
-
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
Clostridium tetanomorphum NCIMB 11547
-
 NCIMB 11547
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrobacter amalonaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Clostridium tetanomorphum
additional information
-
-
 Citrobacter amalonaticus
179
-
-
 Citrobacter amalonaticus
282
-
 incubation with 5 mM EDTA, pH 7.0, 4¦C, for 3 h Citrobacter amalonaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3R)-3-ethylaspartic acid
-
 Clostridium tetanomorphum ?
-
 ?
(2S,3R)-3-ethylaspartic acid
-
 Citrobacter amalonaticus ?
-
 ?
(2S,3S)-3-ethylaspartic acid
-
 Clostridium tetanomorphum ?
-
 ?
(2S,3S)-3-ethylaspartic acid
-
 Citrobacter amalonaticus ?
-
 ?
3-chloroaspartate
-
 Clostridium tetanomorphum chlorofumarate + NH3
-
 ?
3-chloroaspartate (2R,3S)-3-chloroaspartic acid Citrobacter amalonaticus chlorofumarate + NH3
-
 ?
3-chloroaspartate (2R,3S)-3-chloroaspartic acid Citrobacter amalonaticus YG-1002 chlorofumarate + NH3
-
 ?
3-chloroaspartate
-
 Clostridium tetanomorphum H1 / ATCC 15920 chlorofumarate + NH3
-
 ?
3-chloroaspartate
-
 Clostridium tetanomorphum NCIMB 11547 chlorofumarate + NH3
-
 ?
ethylfumarate + NH3
-
 Clostridium tetanomorphum ?
-
 ?
ethylfumarate + NH3
-
 Citrobacter amalonaticus ?
-
 ?
ethylfumarate + NH3
-
 Citrobacter amalonaticus YG-1002 ?
-
 ?
ethylfumarate + NH3
-
 Clostridium tetanomorphum H1 / ATCC 15920 ?
-
 ?
ethylfumarate + NH3
-
 Clostridium tetanomorphum NCIMB 11547 ?
-
 ?
L-threo-3-methylaspartate r Clostridium tetanomorphum mesaconate + NH3
-
 ?
L-threo-3-methylaspartate r Citrobacter amalonaticus mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter amalonaticus mesaconate + NH3
-
 ?
L-threo-3-methylaspartate r Citrobacter amalonaticus YG-1002 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter amalonaticus YG-1002 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate r Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate r Clostridium tetanomorphum NCIMB 11547 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum NCIMB 11547 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate
-
 Clostridium tetanomorphum ?
-
 ?
L-threo-3-methylaspartate
-
 Citrobacter amalonaticus ?
-
 ?
additional information
-
 Clostridium tetanomorphum ?
-
 ?
additional information
-
 Citrobacter amalonaticus ?
-
 ?
additional information
-
 Citrobacter amalonaticus YG-1002 ?
-
 ?
additional information
-
 Clostridium tetanomorphum H1 / ATCC 15920 ?
-
 ?
additional information
-
 Clostridium tetanomorphum NCIMB 11547 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Clostridium tetanomorphum
dimer 2 * 42000, SDS-PAGE Citrobacter amalonaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 maximal activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 6% loss of activity Citrobacter amalonaticus
50
-
 0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 10% loss of activity Citrobacter amalonaticus
55
-
 0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 44% loss of activity Citrobacter amalonaticus
60
-
 0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methxylaspartate, 96.5% loss of activity Citrobacter amalonaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate Citrobacter amalonaticus
9.7
-
 maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate Citrobacter amalonaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
-
 Citrobacter amalonaticus
5.5 11 30¦C, 10 min, deamination reaction of L-threo-3-methylaspartate, 14% loss of activity Citrobacter amalonaticus