Literature summary for 4.3.1.2 extracted from

Pollard, J.R.; Richardson, S.; Akhtar, M.; Lasry, P.; Neal, T.; Botting, N.P.; Gani, D.
Mechanism of 3-methylaspartase probed using deuterium and solvent isotope effects and active-site directed reagents: identification of an essential cysteine residue (1999), Bioorg. Med. Chem., 7, 949-975.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Clostridium tetanomorphum

Inhibitors

Inhibitors	Comment	Organism	Structure
4-nitrophenylhydrazine	90% inhibition after 200 min	Clostridium tetanomorphum
methylhydrazine	-	Clostridium tetanomorphum
N-ethylmaleimide	1 mM, complete inactivation after approx. 12 min	Clostridium tetanomorphum
phenylhydrazine	20 mM, complete inhibition after 20 min	Clostridium tetanomorphum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5.2	-	(2S,3R)-3-methylaspartate	30°C, pH 9.0, at 50 mM K+	Clostridium tetanomorphum
40	-	(2S,3R)-3-methylaspartate	30°C, pH 9.0, at 1 mM K+	Clostridium tetanomorphum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	required for activity	Clostridium tetanomorphum
Mg2+	required for activity	Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-threo-3-methylaspartate	Clostridium tetanomorphum	-	mesaconate + NH3	-	r
L-threo-3-methylaspartate	Clostridium tetanomorphum H1 / ATCC 15920	-	mesaconate + NH3	-	r

Organism

Organism	UniProt	Comment	Textmining
Clostridium tetanomorphum	-	-	-
Clostridium tetanomorphum H1 / ATCC 15920	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native and recombinant 3-methylaspartase	Clostridium tetanomorphum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
erythro-3-methylaspartate	-	Clostridium tetanomorphum	?	-	?
erythro-3-methylaspartate	-	Clostridium tetanomorphum H1 / ATCC 15920	?	-	?
L-threo-3-methylaspartate	-	Clostridium tetanomorphum	mesaconate + NH3	-	r
L-threo-3-methylaspartate	-	Clostridium tetanomorphum H1 / ATCC 15920	mesaconate + NH3	-	r

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Release 2023.1 (January 2023)