Literature summary for 4.3.1.2 extracted from

Raj, H.; Weiner, B.; Veetil, V.P.; Reis, C.R.; Quax, W.J.; Janssen, D.B.; Feringa, B.L.; Poelarends, G.J.
Alteration of the diastereoselectivity of 3-methylaspartate ammonia lyase by using structure-based mutagenesis (2009), ChemBioChem, 10, 2236-2245.

Search for more literature for 4.3.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Clostridium tetanomorphum

Protein Variants

Protein Variants	Comment	Organism
H194A	enhanced diastereoselectivities	Clostridium tetanomorphum
K331A	enhanced diastereoselectivities	Clostridium tetanomorphum
Q329A	enhanced diastereoselectivities	Clostridium tetanomorphum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.7	-	mesaconic acid	wild-type	Clostridium tetanomorphum
1	-	L-threo-3-methylaspartic acid	wild-type	Clostridium tetanomorphum
1	-	L-threo-3-methylaspartic acid	mutant Q329A	Clostridium tetanomorphum
1.7	-	mesaconic acid	mutant Q329A	Clostridium tetanomorphum
1.8	-	L-threo-3-methylaspartic acid	mutant H194A	Clostridium tetanomorphum
14	-	mesaconic acid	mutant H194A	Clostridium tetanomorphum

Organism

Organism	UniProt	Comment	Textmining
Clostridium tetanomorphum	Q05514	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-threo-3-methylaspartic acid	assay at pH 9.0, 30°C	Clostridium tetanomorphum	mesaconate + NH3	-	?
mesaconic acid + NH3	assay at pH 9.0, 30°C	Clostridium tetanomorphum	L-threo-3-methylaspartate	-	r

Synonyms

Synonyms	Comment	Organism
3-methylaspartate ammonia lyase	-	Clostridium tetanomorphum
MAL	-	Clostridium tetanomorphum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Clostridium tetanomorphum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	L-threo-3-methylaspartic acid	mutant Q329A	Clostridium tetanomorphum
0.25	-	mesaconic acid	mutant Q329A	Clostridium tetanomorphum
0.55	-	L-threo-3-methylaspartic acid	mutant H194A	Clostridium tetanomorphum
8.4	-	mesaconic acid	mutant H194A	Clostridium tetanomorphum
61	-	mesaconic acid	wild-type	Clostridium tetanomorphum
89	-	L-threo-3-methylaspartic acid	wild-type	Clostridium tetanomorphum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	assay at	Clostridium tetanomorphum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	mesaconic acid	mutant K331A, value below 0.000001	Clostridium tetanomorphum
additional information	-	L-threo-3-methylaspartic acid	mutant K331A, value below 0.000001	Clostridium tetanomorphum
0.01	-	L-threo-3-methylaspartic acid	mutant Q329A	Clostridium tetanomorphum
0.15	-	mesaconic acid	mutant Q329A	Clostridium tetanomorphum
0.31	-	L-threo-3-methylaspartic acid	mutant H194A	Clostridium tetanomorphum
0.6	-	mesaconic acid	mutant H194A	Clostridium tetanomorphum
87	-	mesaconic acid	wild-type	Clostridium tetanomorphum
89	-	L-threo-3-methylaspartic acid	wild-type	Clostridium tetanomorphum

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Release 2023.1 (January 2023)