Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Clostridium tetanomorphum |
Protein Variants | Comment | Organism |
---|---|---|
C361A | kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 47% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) | Clostridium tetanomorphum |
C361K | mutant shows no reactivity. Conversion 2h: 10% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) | Clostridium tetanomorphum |
F170A | kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 63% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) | Clostridium tetanomorphum |
L384A | kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 61% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) | Clostridium tetanomorphum |
Q172A | kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 16% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) | Clostridium tetanomorphum |
Q172N | mutant shows no reactivity. Conversion 2h: 6% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) | Clostridium tetanomorphum |
Q73A | kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 37% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 91:9 (89:11 wild-type) | Clostridium tetanomorphum |
Q73N | mutant shows very low-level amination activity preventing the measurement of kinetic parameters. Conversion 2h: 18% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) | Clostridium tetanomorphum |
T360A | kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 38% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 93:7 (89:11 wild-type) | Clostridium tetanomorphum |
T360S | kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 76% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 86:14 (89:11 wild-type) | Clostridium tetanomorphum |
Y356A | kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 24% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) | Clostridium tetanomorphum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
mesaconate | pH 9, 30°C, mutant Q73A | Clostridium tetanomorphum | |
0.7 | - |
mesaconate | pH 9, 30°C, wild-type | Clostridium tetanomorphum | |
0.9 | - |
mesaconate | pH 9, 30°C, mutant F170A | Clostridium tetanomorphum | |
1.4 | - |
mesaconate | pH 9, 30°C, mutant T360S | Clostridium tetanomorphum | |
2.9 | - |
mesaconate | pH 9, 30°C, mutant C361A | Clostridium tetanomorphum | |
15 | - |
mesaconate | pH 9, 30°C, mutant T360A | Clostridium tetanomorphum | |
60 | - |
mesaconate | pH 9, 30°C, mutant L384A | Clostridium tetanomorphum | |
60 | - |
mesaconate | pH 9, 30°C, mutant Q172A | Clostridium tetanomorphum | |
60 | - |
mesaconate | pH 9, 30°C, mutant Y356A | Clostridium tetanomorphum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetanomorphum | Q05514 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium tetanomorphum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mesaconate + NH3 | conversion 2h: 73% (wild-type), diastereomeric product ratio (threo:erythro) after 2h: 89:11 | Clostridium tetanomorphum | L-threo-3-methylaspartate + L-erythro-3-methylaspartate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
MAL | - |
Clostridium tetanomorphum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Clostridium tetanomorphum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
mesaconate | pH 9, 30°C, mutant T360A | Clostridium tetanomorphum | |
0.7 | - |
mesaconate | pH 9, 30°C, mutant Q73A | Clostridium tetanomorphum | |
0.7 | - |
mesaconate | pH 9, 30°C, mutant Y356A | Clostridium tetanomorphum | |
10 | - |
mesaconate | pH 9, 30°C, mutant F170A | Clostridium tetanomorphum | |
16 | - |
mesaconate | pH 9, 30°C, mutant L384A | Clostridium tetanomorphum | |
20 | - |
mesaconate | pH 9, 30°C, mutant Q172A | Clostridium tetanomorphum | |
27.5 | - |
mesaconate | pH 9, 30°C, mutant C361A | Clostridium tetanomorphum | |
35 | - |
mesaconate | pH 9, 30°C, mutant T360S | Clostridium tetanomorphum | |
61 | - |
mesaconate | pH 9, 30°C, wild-type | Clostridium tetanomorphum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Clostridium tetanomorphum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
mesaconate | pH 9, 30°C, mutant T360A | Clostridium tetanomorphum | |
0.27 | - |
mesaconate | pH 9, 30°C, mutant L384A | Clostridium tetanomorphum | |
0.342 | - |
mesaconate | pH 9, 30°C, mutant Q172A | Clostridium tetanomorphum | |
3.5 | - |
mesaconate | pH 9, 30°C, mutant Q73A | Clostridium tetanomorphum | |
9.5 | - |
mesaconate | pH 9, 30°C, mutant C361A | Clostridium tetanomorphum | |
11 | - |
mesaconate | pH 9, 30°C, mutant F170A | Clostridium tetanomorphum | |
12 | - |
mesaconate | pH 9, 30°C, mutant Y356A | Clostridium tetanomorphum | |
25 | - |
mesaconate | pH 9, 30°C, mutant T360S | Clostridium tetanomorphum | |
87 | - |
mesaconate | pH 9, 30°C, wild-type | Clostridium tetanomorphum |