Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminoindane-2-phosphonate | - |
Ustilago maydis | |
2-mercaptoethanol | above 25 mM | Ustilago maydis | |
8-hydroxyquinoline | - |
Ustilago maydis | |
Ag+ | - |
Ustilago maydis | |
Cinnamic acid | trans-cinnamate, moderately; trans-cinnamic acid | Ustilago maydis | |
CN- | NaCN | Ustilago maydis | |
Cu2+ | - |
Ustilago maydis | |
Hg2+ | - |
Ustilago maydis | |
NaBH4 | - |
Ustilago maydis | |
p-chloromercuribenzoate | - |
Ustilago maydis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
L-Phe | - |
Ustilago maydis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for metal ion cofactors | Ustilago maydis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
4 * 80000, SDS-PAGE | Ustilago maydis |
320000 | - |
non-denaturing PAGE | Ustilago maydis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ustilago maydis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | no glycosylation | Ustilago maydis |
Purification (Comment) | Organism |
---|---|
- |
Ustilago maydis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Ustilago maydis |
Storage Stability | Organism |
---|---|
4°C, 5% loss of activity after 1 week, 40% loss of activity after 1 month | Ustilago maydis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe | - |
Ustilago maydis | (E)-cinnamate + NH3 | - |
? | |
L-tyrosine | no deamination of L-Tyr | Ustilago maydis | p-coumarate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 80000, SDS-PAGE | Ustilago maydis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Ustilago maydis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.0, 10 min, stable | Ustilago maydis |
60 | - |
pH 7.0, 10 min, 30% loss of activity | Ustilago maydis |
70 | - |
pH 7.0, 10 min, complete loss of activity | Ustilago maydis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | 9.2 | - |
Ustilago maydis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 9.2 | pH 6.5: 50% of maximal activity, pH 8.8-9.2: optimum | Ustilago maydis |