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Literature summary for 4.3.1.24 extracted from

  • Moffitt, M.C.; Louie, G.V.; Bowman, M.E.; Pence, J.; Noel, J.P.; Moore, B.S.
    Discovery of two cyanobacterial phenylalanine ammonia lyases: kinetic and structural characterization (2007), Biochemistry, 46, 1004-1012.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trichormus variabilis
expression in Escherichia coli Nostoc punctiforme

Crystallization (Commentary)

Crystallization (Comment) Organism
space group I222, 2.5 A resolution. Enzyme exists as homotetramer. Comparison with enzyme from Anabaena variabilis Nostoc punctiforme
space group P1, 1.9 A resolution. Enzyme exists as homotetramer. Comparison with enzyme from Nostoc punctiforme Trichormus variabilis

Protein Variants

Protein Variants Comment Organism
L108A slight decrease in catalytic activity Trichormus variabilis
L108A slight decrease in catalytic activity Nostoc punctiforme
L108G slight decrease in catalytic activity Trichormus variabilis
L108G slight decrease in catalytic activity Nostoc punctiforme
additional information mutant lacking 21 N-terminal amino acids, no adverse effects on catalytic activity Trichormus variabilis
additional information mutant lacking 21 N-terminal amino acids, no adverse effects on catalytic activity Nostoc punctiforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 L-phenylalanine wild-type, pH 8.5, 37°C Nostoc punctiforme
0.06
-
 L-phenylalanine wild-type, pH 8.5, 37°C Trichormus variabilis
0.06
-
 L-phenylalanine mutant lacking 21 N-terminal amino acids, pH 8.5, 37°C Trichormus variabilis
0.069
-
 L-phenylalanine mutant L108A, pH 8.5, 37°C Trichormus variabilis
0.07
-
 L-phenylalanine mutant L108G, pH 8.5, 37°C Trichormus variabilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 4 * 64000, SDS-PAGE, recombinant enzyme with His-tag, and crystallization data Trichormus variabilis
64000
-
 4 * 64000, SDS-PAGE, recombinant enzyme with His-tag, and crystallization data Nostoc punctiforme

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme
-
 strain ATCC 29133
-
Trichormus variabilis
-
 strain ATCC 29415
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine
-
 Trichormus variabilis (E)-cinnamate + NH3
-
 ?
L-phenylalanine
-
 Nostoc punctiforme (E)-cinnamate + NH3
-
 ?
L-tyrosine poor substrate Trichormus variabilis p-coumarate + NH3
-
 ?
additional information no substrate: L-histidine Trichormus variabilis ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 64000, SDS-PAGE, recombinant enzyme with His-tag, and crystallization data Trichormus variabilis
tetramer 4 * 64000, SDS-PAGE, recombinant enzyme with His-tag, and crystallization data Nostoc punctiforme

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Trichormus variabilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.95
-
 L-phenylalanine mutant L108G, pH 8.5, 37°C Trichormus variabilis
1.96
-
 L-phenylalanine wild-type, pH 8.5, 37°C Nostoc punctiforme
3.6
-
 L-phenylalanine mutant L108A, pH 8.5, 37°C Trichormus variabilis
4.4
-
 L-phenylalanine wild-type, pH 8.5, 37°C Trichormus variabilis
4.6
-
 L-phenylalanine mutant lacking 21 N-terminal amino acids, pH 8.5, 37°C Trichormus variabilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Trichormus variabilis