Cloned (Comment) | Organism |
---|---|
C- or N-terminal His-tagged enzymes are expressed in Escherichia coli Rosetta 2 (DE3) cells | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | competitive inhibition; competitive inhibition; competitive inhibition; competitive inhibition | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0364 | - |
L-phenylalanine | isoform PAL3, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
0.0395 | - |
L-phenylalanine | isoform PAL2, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
0.0524 | - |
L-phenylalanine | isoform PAL4, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
0.0598 | - |
L-phenylalanine | isoform PAL1, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Nicotiana tabacum |
262000 | - |
PAL1 with a C-terminal His-tag, gel filtration | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | C6ZIA5 | isoform PAL4; cultivar Xanthi | - |
Nicotiana tabacum | P25872 | isoform PAL1; cultivar Xanthi | - |
Nicotiana tabacum | P35513 | isoform PAL2; cultivar Xanthi | - |
Nicotiana tabacum | P45733 | isoform PAL3; cultivar Xanthi | - |
Purification (Comment) | Organism |
---|---|
using the MagneHis protein purification system | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
petal | - |
Nicotiana tabacum | - |
pith | - |
Nicotiana tabacum | - |
root | - |
Nicotiana tabacum | - |
stamen | - |
Nicotiana tabacum | - |
stem cortex | - |
Nicotiana tabacum | - |
xylem | - |
Nicotiana tabacum | - |
Storage Stability | Organism |
---|---|
-80°C, storage buffer, several months, no loss of activity | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Nicotiana tabacum | (E)-cinnamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
L-phenylalanine ammonia-lyase | - |
Nicotiana tabacum |
PAL | - |
Nicotiana tabacum |
PAL1 | - |
Nicotiana tabacum |
PAL2 | - |
Nicotiana tabacum |
PAL3 | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
L-phenylalanine | isoform PAL3, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
1.09 | - |
L-phenylalanine | isoform PAL1, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
1.14 | - |
L-phenylalanine | isoform PAL2, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
1.53 | - |
L-phenylalanine | isoform PAL4, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
2-mercaptoethanol | isoform PAL1 | Nicotiana tabacum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Nicotiana tabacum | isoform PAL1 with His-tag, isoelectric focusing | - |
6.8 |
Organism | Comment | Expression |
---|---|---|
Nicotiana tabacum | expression of PAL transcripts peak 4 h after exposure to 0.05 mg/ml yeast elicitor, whereas 0.025 mM methyl jasmonate induction of PAL transcripts is slower | up |
General Information | Comment | Organism |
---|---|---|
metabolism | L-phenylalanine ammonia-lyase is the first enzyme of phenylpropanoid biosynthesis | Nicotiana tabacum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.28 | - |
L-phenylalanine | isoform PAL1, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
21.1 | - |
L-phenylalanine | isoform PAL3, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
28.93 | - |
L-phenylalanine | isoform PAL2, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum | |
29.09 | - |
L-phenylalanine | isoform PAL4, at 30°C in 50 mM Tris/HCl, pH 8.5 | Nicotiana tabacum |